Restraint Stress Modulation of the Skin Immune Response Is Independent of T Cell Proliferation

Details

• Personal Author:
  Depree KM ; Flint MS ; Tinkle SS
• Description:
  Previous reports suggest that restraint stress applied to sensitized mice before challenge enhances the mouse ear swelling response. To determine if this event was localized to the skin and /or the lymph nodes, we assessed Langerhans cell (LC) morphology by analyzing FITC-conjugated Ia stained cells in epidermal sheets and cell proliferation by the Local Lymph Node Assay. We determined that stress induced significant alterations in LC morphology and decreased DNFB-induced lymph node (LN) cell proliferation by 46%. To understand if these suppressive effects were T cell specific, we asked if restraint modulates LN cell surface expression of CD3, CD4, CD8 and CD62L. Using flow cytometry, we found that restraint had no effect on CD3, CD4, or CD8 expression. Although DNFB significantly increased production and staining intensity of CD62L, restraint did not alter DNFB-decreased CD62L expression. We extended these studies to T-cell deficient nude mice and found that stress before challenge enhanced ear swelling in the absence of a specific immune response. These data suggest that stress applied prior to challenge modulates the skin response to chemical but not the specific T cell response in lymph nodes. [Description provided by NIOSH]
• Subjects:
  Animals Immune System Laboratories Occupational Health Safety Skin Skin Diseases
• Keywords:
  Animal-studies Immune-reaction Immune-system Laboratory-animals Lymph-nodes Skin-disorders Skin-exposure Stress
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Louisiana ; OSHA Region 3 ; OSHA Region 6 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  16
• Issue:
  5
• NIOSHTIC Number:
  nn:20023387
• Citation:
  FASEB J 2002 Mar; 16(5):A1058-A1059
• Contact Point Address:
  NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
• Federal Fiscal Year:
  2002
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal, Experimental Biology 2002, New Orleans, Louisiana, April 20-24, 2002
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1a63b2334d1b01e8ab1583db53a6ee82316e7f3c7d9a01498c51ae8a9b63aef8672919e25d23c2e35f74644cc4d90f65ce659380804f77149fe40e8ad2f0e8dc
• Download URL:
  https://stacks.cdc.gov/view/cdc/195847/cdc_195847_DS1.pdf
• File Type:
  [PDF - 484.96 KB ]