Investigation of the pulmonary bioactivity of double-walled carbon nanotubes

Details

• Personal Author:
  Andrew M ; Battelli LA ; Castranova, Vincent ; Endo M ; Leonard, Stephen S. ; Porter DW ; Sager TM ; Steinbach T ; Tsuruoka S ; Wolfarth MW
• Description:
  Double-walled carbon nanotubes (DWCNT) are a rather new and unexplored variety of carbon nanotubes. Previously conducted studies established that exposure to a variety of carbon nanotubes produced lung inflammation and fibrosis in mice after pharyngeal aspiration. However, the bioactivity of double-walled carbon nanotubes (DWCNT) has not been determined. In this study, the hypothesis that DWCNT would induce pulmonary toxicity was explored by analyzing the pulmonary bioactivity of DWCNT. To test this hypothesis, C57Bl/6 mice were exposed to DWCNT by pharyngeal aspiration.Mice underwent whole-lung lavage (WLL) to assess pulmonary inflammation and injury, and lung tissue was examined histologically for development of pulmonary disease as a function of dose and time. The results showed that DWCNT exposure produced a dose-dependent increase in WLL polymorphonuclear leukocytes (PMN), indicating that DWCNT exposure initiated pulmonary inflammation. DWCNT exposure also produced a dose-dependent rise in lactate dehydrogenase (LDH) activity, as well as albumin levels, in WLL fluid, indicating that DWCNT exposure promoted cytotoxicity as well as decreases in the integrity of the blood-gas barrier in the lung, respectively. In addition, at 7 and 56 d postexposure, the presence of significant alveolitis and fibrosis was noted in mice exposed to 40 ug/mouse DWCNT. In conclusion, this study provides insight into previously uninvestigated pulmonary bioactivity of DWCNT exposure. Data indicate that DWCNT exposure promotes inflammation, injury, and fibrosis in the lung. [Description provided by NIOSH]
• Subjects:
  Animals Cytotoxins Fibrosis Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Risk Assessment Risk Factors Safety

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• Keywords:
  Cytotoxicity Exposure-levels Laboratory-animals Lung-disorders Lung-function Lung-tissue Pulmonary-function Pulmonary-system Pulmonary-system-disorders Risk-factors
• ISSN:
  1528-7394
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Virginia ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  76
• Issue:
  15
• NIOSHTIC Number:
  nn:20043312
• Citation:
  J Toxicol Environ Health A 2013 Aug; 76(15):922-936
• Contact Point Address:
  Tina M. Sager, PhD, National Institute for Occupational Safety and Health, 1095 Willowdale Road, M/S 2015, Morgantown, WV 26505
• Email:
  sst2@cdc.gov
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cea23e2d99945a273bf49b1aadfbb56240323630a1f17eec0387ddf41a5a541c30703202b3891087f3634b421e4b1cb9edfa6e2b49734d74ab708c8b5408b5e8
• Download URL:
  https://stacks.cdc.gov/view/cdc/195237/cdc_195237_DS1.pdf
• File Type:
  [PDF - 786.90 KB ]