A microfluidic paper-based analytical device for rapid quantification of particulate chromium

Details

• Personal Author:
  Cate DM ; Chailapakul O ; Dungchai W ; Henry CS ; Rattanarat P ; Siangproh W ; Volckens J
• Description:
  Occupational exposure to Cr is concerning because of its myriad of health effects. Assessing chromium exposure is also cost and resource intensive because the analysis typically uses sophisticated instrumental techniques like inductively coupled plasma-mass spectrometry (ICP-MS). Here, we report a novel, simple, inexpensive microfluidic paper-based analytical device (µPAD) for measuring total Cr in airborne particulate matter. In the µPAD, tetravalent cerium (Ce(IV)) was used in a pretreatment zone to oxidize all soluble Cr to Cr(VI). After elution to the detection zone, Cr(VI) reacts with 1,5-diphenylcarbazide (1,5-DPC) forming 1,5-diphenylcarbazone (DPCO) and Cr(III). The resulting Cr(III) forms a distinct purple colored complex with the DPCO. As proof-of-principle, particulate matter (PM) collected on a sample filter was analyzed with the µPAD to quantify the mass of total Cr. A log-linear working range (0.23-3.75µg; r(2)=0.998) between Cr and color intensity was obtained with a detection limit of 0.12µg. For validation, a certified reference containing multiple competing metals was analyzed. Quantitative agreement was obtained between known Cr levels in the sample and the Cr measured using the µPAD. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Chemistry Chemistry, Analytic Chromium Metals Occupational Health Particulate Matter Safety
• Keywords:
  Airborne-particles Analytical-chemistry Analytical-instruments Analytical-processes Author Keywords: Paper-based Analytical Device Chromium-compounds Filters Fluids Metal-compounds Metallic-compounds Microfluidics Oxidizers Particulate-dust Particulate-sampling-methods Particulate Matter Particulates Quantitative-analysis Sample-preparation

  More +
• ISSN:
  0003-2670
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Colorado ; OSHA Region 8
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  50-55
• Volume:
  800
• NIOSHTIC Number:
  nn:20043247
• Citation:
  Anal Chim Acta 2013 Oct; 800:50-55
• Contact Point Address:
  John Volckens, Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, CO 80523
• Email:
  john.volckens@colostate.edu
• CAS Registry Number:
  16065-83-1 ; Chromic trioxide (CAS RN 1333-82-0) ; Chromium (CAS RN 7440-47-3) ; Chromium(6+) (CAS RN 18540-29-9)
• Federal Fiscal Year:
  2014
• Performing Organization:
  Colorado State University - Fort Collins
• Peer Reviewed:
  True
• Start Date:
  20110701
• Source Full Name:
  Analytica Chimica Acta
• End Date:
  20130630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d4d74befe78ca0a73a6e95d43b89b750b82657b34216622c6d3c65952a00a2ba3bb7e7f5b6dc6985da076b646c2578b5a7f02684b421c5fafd3a3a6ff306936e
• Download URL:
  https://stacks.cdc.gov/view/cdc/195188/cdc_195188_DS1.pdf
• File Type:
  [PDF - 1.48 MB ]