Lung tumor promotion by chromium-containing welding particulate matter in a mouse model

Details

• Personal Author:
  Antonini JM ; Battelli LA ; Erdely A ; Kashon ML ; Keane M ; Meighan TG ; Zeidler-Erdely PC
• Description:
  Background: Epidemiology suggests that occupational exposure to welding particulate matter (PM) may increase lung cancer risk. However, animal studies are lacking to conclusively link welding with an increased risk. PM derived from stainless steel (SS) welding contains carcinogenic metals such as hexavalent chromium and nickel. We hypothesized that welding PM may act as a tumor promoter and increase lung tumor multiplicity in vivo. Therefore, the capacity of chromium-containing gas metal arc (GMA)-SS welding PM to promote lung tumors was evaluated using a two-stage (initiation-promotion) model in lung tumor susceptible A/J mice. Methods: Male mice (n = 28-30/group) were treated either with the initiator 3-methylcholanthrene (MCA;10 ug/g; IP) or vehicle (corn oil) followed by 5 weekly pharyngeal aspirations of GMA-SS (340 or 680 ug/exposure) or PBS. Lung tumors were enumerated at 30 weeks post-initiation. Results: MCA initiation followed by GMA-SS welding PM exposure promoted tumor multiplicity in both the low (12.1+/- 1.5 tumors/mouse) and high (14.0 +/- 1.8 tumors/mouse) exposure groups significantly above MCA/sham (4.77 +/- 0.7 tumors/mouse; p = 0.0001). Multiplicity was also highly significant (p < 0.004) across all individual lung regions of GMA-SS-exposed mice. No exposure effects were found in the corn oil groups at 30 weeks. Histopathology confirmed the gross findings and revealed increased inflammation and a greater number of malignant lesions in the MCA/welding PM-exposed groups. Conclusions: GMA-SS welding PM acts as a lung tumor promoter in vivo. Thus, this study provides animal evidence to support the epidemiological data that show welders have an increased lung cancer risk. [Description provided by NIOSH]
• Subjects:
  Animals Carcinogens Chromium Epidemiology Laboratories Lung Metals Neoplasms Nickel Occupational Health Respiratory System Risk Assessment Risk Factors Safety Welding

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• Keywords:
  Author Keywords: A/J Mouse Cancer Chromium-compounds Exposure-levels Laboratory-animals Metallic-compounds Parasiticides Risk-factors Tumors Welders
• ISSN:
  1743-8977
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  45
• Volume:
  10
• NIOSHTIC Number:
  nn:20043211
• Citation:
  Part Fibre Toxicol 2013 Sep; 10:45
• Contact Point Address:
  Patti C Zeidler-Erdely, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road MS L2015, Morgantown, WV 26505
• Email:
  paz9@cdc.gov
• CAS Registry Number:
  3-Methylcholanthrene (CAS RN 56-49-5) ; Chromium(6+) (CAS RN 18540-29-9) ; Nickel (CAS RN 7440-02-0)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Construction
• Peer Reviewed:
  True
• Source Full Name:
  Particle and Fibre Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:63417f526ece81961ced6eee1d7db22ba06539811e1de8f6f1f588008d98a4597699d529aea5f31c133a3a8619a92d411b07d70b354d109b571b7b76d4eb30aa
• Download URL:
  https://stacks.cdc.gov/view/cdc/195175/cdc_195175_DS1.pdf
• File Type:
  [PDF - 1.15 MB ]