Transport of inhaled MWCNT to the pleura, respiratory muscles and systemic organs

Details

• Personal Author:
  Battelli LA ; Castranova, Vincent ; Hubbs, Ann F. ; Mercer RR ; Porter DW ; Scabilloni JF ; Wang L
• Description:
  Inhalation exposure studies of mice were conducted to determine if multi-walled carbon nanotubes (MWCNT) distribute to the parietal pleura, respiratory musculature and systemic organs. Male C57BL/6J mice were exposed in a whole-body inhalation system to a 5 mg/m3 MWCNT aerosol for 5 hours/day for 12 days (4 times/week for 3 weeks). At 1 day and 48 weeks after the 12 day exposure period, mice were anesthetized and lungs and systemic tissues were preserved by whole body vascular perfusion of paraformaldehyde while inflated with air. A separate, clean-air control group was studied. Sirius Red stained sections from lung, diaphragm, chest wall, heart, kidney and liver were analyzed. Enhanced darkfield microscopy and morphometric methods were used to detect and count MWCNT in tissue sections. Counts in tissue sections were expressed as number of MWCNT per cm2 of tissue (mean+/-SE, N=8 mice per group). Although agglomerates account for approximately 60% of lung burden, only singlet MWCNT were observed in diaphragm, chest wall and systemic tissues. At one day post exposure, the average length of singlet MWCNT in diaphragm was comparable to that of singlet MWCNT in the lungs 5.6 +/- 0.6 versus 5.1 +/- 0.6 um, respectively. There were 26 +/- 13 and 134 +/- 25 per cm2 in tissue sections of diaphragm at 1 day and 48 weeks post exposure, respectively. On average, there were 18 +/- 5 and 50 +/- 20 per cm2 singlet MWCNT observed in systemic organ tissue sections at 1 day and 48 weeks, respectively. The burden of singlet MWCNT in parietal pleura, respiratory musculature and systemic organs at 48 weeks post exposure was significantly higher than at 1 day post exposure. Results demonstrate that inhaled MWCNT, which deposit in the lungs, are transported to the parietal pleura, the respiratory musculature and the systemic organs in a singlet form and accumulate with time following exposure. [Description provided by NIOSH]
• Subjects:
  Aerosols Environmental Monitoring Laboratories Lung Musculoskeletal System Occupational Health Particulate Matter Respiratory System Safety Toxicology
• Keywords:
  Dose-response Exposure-assessment Exposure-levels Fiber-deposition Heart Histopathology Inhalation-studies Kidneys Laboratory-animals Laboratory-techniques Liver Liver-tissue Lung-burden Lung-tissue Muscle-tissue Nanotechnology Particle-counters Pleural-cavity Pulmonary-system Tissue-culture

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; OSHA Region 6 ; Texas ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  96-97
• Volume:
  132
• Issue:
  1
• NIOSHTIC Number:
  nn:20042398
• Citation:
  Toxicologist 2013 Mar; 132(1):96-97
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2013
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 52nd Annual Meeting and ToxExpo, March 10-14, 2013, San Antonio, Texas
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:be733e9c85468a163fa2ead90d91cba99a01adae967d549836f84dbb1926622a1065acb69e935e955befab9e385a16cd058cd119dd6ba520212eb39438427a42
• Download URL:
  https://stacks.cdc.gov/view/cdc/194630/cdc_194630_DS1.pdf
• File Type:
  [PDF - 593.31 KB ]