Simultaneous determination of multiple phthalate metabolites and bisphenol-A in human urine by liquid chromatography-tandem mass spectrometry

Details

• Personal Author:
  Austin C ; Chen M ; Collins EM ; Lu C ; Tao L
• Description:
  Phthalates and bisphenol A are environmental endocrine-disrupting chemicals used widely in common consumer products. There is increasing concern about human exposure to phthalates and bisphenol A due to the potential adverse effects related to the anti-androgenic activity of phthalates and estrogenic activity of bisphenol A. In assessing environmental exposure to phthalates and bisphenol A, it is essential to have a validated analytical method that can quantify trace concentrations of phthalate metabolites and bisphenol A in humans. In this study, we developed and validated an accurate, sensitive, and robust LC-MS/MS method to simultaneously quantify 5 phthalate monoester metabolites, including mono-methyl phthalate, mono-ethyl phthalate, mono-butyl phthalate, mono-benzyl phthalate, mono- 2-ethylhexyl phthalate, and bisphenol A in human urine. In this method, the phthalate metabolites and bisphenol A, along with their isotope labeled internal standards, were extracted from 200 ul of human urine using automated off-line solid phase extraction. The analytes were quantitatively determined using LC-MS/MS operated in negative electrospray ionization multiple reaction-monitoring mode. The limit of quantification was 0.3 ng/ml for mono-methyl phthalate, mono-ethyl phthalate, mono-benzyl phthalate and bisphenol A, and 1 ng/ml for mono-butyl phthalate and mono-2-ethylhexyl phthalate. The precision and accuracy were well within the acceptable 15% range. This validated method has been used successfully in assessing exposure to phthalates and bisphenol A in humans. [Description provided by NIOSH]
• Subjects:
  Chemistry Energy Metabolism Environmental Exposure Estrogens Men Occupational Health Phthalic Acids Safety Urine Women
• Keywords:
  Analytical-methods Analytical-processes Author Keywords: Phthalate Metabolite Bisphenol A Chemical-composition Chemical-properties Environmental-exposure Estrogenic-hormones Exposure-levels Humans LC-MS/MS Metabolites Phthalates SPE Urinalysis

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• ISSN:
  1570-0232
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Connecticut ; Massachusetts ; OSHA Region 1
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  73-80
• Volume:
  904
• NIOSHTIC Number:
  nn:20042222
• Citation:
  J Chromatogr B 2012 Sep; 904:73-80
• Contact Point Address:
  Chensheng Lu, Department of Environmental Health, Harvard School of Public Health, 677 Huntington Avenue, Boston, MA 02115
• Email:
  cslu@hsph.harvard.edu
• CAS Registry Number:
  2,2-Bis(4-hydroxyphenyl)propane (CAS RN 80-05-7)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Services
• Performing Organization:
  University of Connecticut Schools of Medicine, Farmington
• Peer Reviewed:
  True
• Start Date:
  20090901
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• End Date:
  20140831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f8f424a2adac99e5b55ecdc9a1e035d130401991a4c1de6dbca28a64713e12376bf00df73a85b90a76db9b0cdc8eeb31252a80418113cfb70b7ebac61ee6c5ef
• Download URL:
  https://stacks.cdc.gov/view/cdc/194520/cdc_194520_DS1.pdf
• File Type:
  [PDF - 388.32 KB ]