Bioconcentration, metabolism and excretion of triclocarban in larval Qurt medaka (Oryzias latipes)

Details

• Personal Author:
  Flores I ; Franze B ; Hammock BD ; Kurobe T ; Ranganathan A ; Schebb NH ; Teh SJ
• Description:
  The antimicrobial triclocarban (TCC) is frequently found in personal care products and commonly observed in surface waters and sediments. Due to its long environmental persistence TCC accumulates in sewage sludge. It also shows a high unintended biological activity as a potent inhibitor of the soluble epoxide hydrolase (sEH) and may be an endocrine disruptor. In this study, we investigated bioconcentration, metabolism and elimination of TCC in fish using medaka (Oryzias latipes) as a model. Medaka larvae (7 +/- 1 days post hatching) were exposed to 63 nM (20 µg/L) TCC water for 24h. The LC-MS/MS analysis of water and tissues provided bioconcentration of TCC and its metabolites in fish body and rapid excretion into culture water. Results from tissue samples showed a tissue concentration of 34 µmol/kg and a log bioconcentration factor (BCF) of 2.86. These results are slightly lower than previous findings in snails and algae. A significant portion of the absorbed TCC was oxidatively metabolized by the fish to hydroxylated products. These metabolites underwent extensive phase II metabolism to yield sulfate and glucuronic acid conjugates. The most abundant metabolite in fish tissue was the glucuronide of 2'-OH-TCC. Elimination of TCC after transferring the fish to fresh water was rapid, with a half-life of 1h. This study shows that larval medaka metabolize TCC similarly to mammals. The rapid rate of metabolism results in a lower bioconcentration than calculated from the octanol-water coefficient of TCC. [Description provided by NIOSH]
• Subjects:
  Endocrine System Environmental Pollution Occupational Health Safety
• Keywords:
  Author Keywords: Triclocarban Bioconcentration Endocrine-function Environmental Contamination Liquid Chromatography-electrospray Tandem Mass Spectrometry Online-solid Phase Extraction Pollutants Pollution Qurt Medaka (Oryzias Latipes) Tissue-culture Triclocarban Metabolites

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• ISSN:
  0166-445X
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  448-454
• Volume:
  105
• Issue:
  3
• NIOSHTIC Number:
  nn:20041192
• Citation:
  Aquat Toxicol 2011 Oct; 105(3-4):448-454
• Contact Point Address:
  Swee J. Teh, University of California, Davis, Aquatic Health Program, Department of Anatomy, Physiology and Cell Biology, School of Veterinary Medicine, One Shields Avenue, Davis, CA 95616
• CAS Registry Number:
  Triclocarban (CAS RN 101-20-2)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Aquatic Toxicology
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0016474f23b4451870a26185b6c888327c2be1d87a5a10d6e566b0465204b338a9091a08a92d95f7c8fec620219ba2b3d4a8fcf08acaa4dfd06f50d5c72f9152
• Download URL:
  https://stacks.cdc.gov/view/cdc/193802/cdc_193802_DS1.pdf
• File Type:
  [PDF - 904.88 KB ]