Biomarkers of occupational exposures to carbon nanotubes in humans

Details

• Personal Author:
  Fatkhutdinova L ; Kagan VE ; Khaliullin T ; Kisin E ; Murray A ; Shvedova A ; Tkach A ; Zalyalov R
• Description:
  Large-scale manufacturing of multiwalled carbon nanotubes (MWCNT) and their wide industrial and biomedical applications raise concerns about potential adverse health effects, especially with respect to pulmonary and systemic outcomes in humans. Of particular importance is the issue of identification of meaningful biomarkers relevant to realistic occupational exposures. We used MWCNT obtained from a manufacturing facility ("NanotechCenter" Ltd., Tambov, Russia) where exposure levels were assessed due to reported excessive exposure of personnel. First, we conducted systematic validation studies using animal and tissue cell models. The dose range was selected based on measured levels of airborne MWCNT at the workplace during various operational procedures (NIOSH 5040 method). Exposure to MWCNT (20-80 microg/mouse) given by pharyngeal aspiration to C57BL/6 mice induced: i) acute inflammation, early granulomatous lesions (7 days) and interstitial fibrosis (28-60 days); ii) increase in biomarkers of inflammation (MPO), pulmonary damage (LDH), fibrosis (TGF-]b, CA125), and oxidative stress (4HNE, protein carbonyls) in bronchoalveolar lavage fluid and serum. Treatment of murine and human airway cells with MWCNT caused dose/time dependent oxidative stress and cytotoxicity. Based on these results - documenting inflammatory and fibrogenic effects of MWCNT - several biomarkers were selected for assessments of health outcomes of exposure to MWCNT. The results of ongoing cross-sectional epidemiological study in which we performed initial assessments of biomarkers of inflammation, early fibrosis and oxidative stress along with psychophysiological tests, cardiovascular and respiratory function assessments in 13 exposed workers are presented. [Description provided by NIOSH]
• Subjects:
  Biomarkers Cytotoxins Environmental Monitoring Hazardous Substances Immune System Laboratories Lung Occupational Exposure Occupational Health Respiratory System Safety

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• Keywords:
  Analytical-Method Author Keywords: Carbon Nanotubes Biological-effects Carbonyls Cell-damage Cell-function Cellular-reactions Cytotoxic-effects Dose-response Employee-exposure Exposure-assessment Exposure-levels Health-hazards Humans Immune-reaction Immune-system Laboratory-animals Laboratory-testing Lung-cells Lung-fibrosis Lung-function Lung-tissue Molecular-biology Nanotechnology Oxidative-processes Proteins Pulmonary-function Pulmonary-system

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  California ; OSHA Region 3 ; OSHA Region 9 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  54
• Volume:
  126
• NIOSHTIC Number:
  nn:20040642
• Citation:
  Toxicologist 2012 Mar; 126(Abstract Suppl):54
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2012
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 51st Annual Meeting and ToxExpo, March 11-15, 2012, San Francisco, California
• Supplement:
  Abstract
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8ff1818a1b1b39a8cf451341c53f07873edda4d824fed09649c0741fb0e2da280eb599bb7ea4d1ffd7b2a93851994f7cee2cac5861ce290ea78d8536ef723223
• Download URL:
  https://stacks.cdc.gov/view/cdc/193433/cdc_193433_DS1.pdf
• File Type:
  [PDF - 530.64 KB ]