Assessment of exposure to PACs in asphalt workers: measurement of urinary PACs and their metabolites with an ELISA kit

Details

• Personal Author:
  Biagini, Raymond E. ; Johnson BC ; Mackenzie BA ; Olsen, Larry D. ; Robertson SA ; Sammons DL ; Smith JP ; Snawder, John E. ; Striley CAF ; Walker CV
• Description:
  An enzyme-linked immunosorbent assay (ELISA) kit made for determination of polycyclic aromatic compounds (PACs) in water was adapted for measuring PACs and their metabolites in urine. This method was then applied to a pilot asphalt worker PAC exposure study. Currently, liquid-liquid extraction with gas chromatography/isotope dilution high-resolution mass spectrometry (GC/HRMS) is the preferred method to determine urinary PAC metabolites. Although sensitive and specific, GC/HRMS is time consuming and costly. The ELISA method had a range from 14-720 ng/ml 1-hydroxypyrene equivalents with a lower limit of detection (LOD) of 14 ng/ml urine. ELISA and GC/HRMS PAC metabolite measurements had a statistically significant correlation and the PAC ELISA results were indicative of potential asphalt exposure. PAC ELISA is promising as a more rapid and less costly routine method for determining worker exposure to PACs in asphalt emissions. [Description provided by NIOSH]
• Subjects:
  Biological Factors Biomarkers Chemistry Energy Metabolism Environmental Monitoring Ethylenes Hydrocarbons Hypersensitivity Immune System Inhalation Exposure Irritants Lung Lung Diseases Occupational Health Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety Urine Workplace

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• Keywords:
  Analytical-processes Asphalt-fumes Asphalt-industry Author Keywords: Asphalt Biological-effects Biological-factors Biological-monitoring Biological-systems Biomonitoring Chemical-hypersensitivity Chemical-properties ELISA Exposure-assessment Immune-reaction Immune-system Immunology Inhalants Lung-disorders Lung-irritants Metabolic-activation Metabolic-study Metabolites PAC Pulmonary-disorders Pulmonary-system Quantitative-analysis Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders Statistical-analysis Urine Urine-chemistry Work-areas Work-operations Work-performance Worker-health Workplace-studies

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• ISSN:
  1040-6638
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Indiana ; Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  270-285
• Volume:
  31
• Issue:
  4
• NIOSHTIC Number:
  nn:20039568
• Citation:
  Polycycl Aromat Compd 2011 Aug; 31(4):270-285
• Contact Point Address:
  Jerome P. Smith, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, 4676 Columbia Pkwy C-26, Cincinnati, OH 45226
• Email:
  JPSmith1@cdc.gov
• Federal Fiscal Year:
  2011
• Peer Reviewed:
  True
• Source Full Name:
  Polycyclic Aromatic Compounds
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0bac6049710f252ba8611a9381606ed267e681a3c72f24e174e551702fa708b4dc9e6ab06b9023badb953ed198f88c5a54a699fa918df4a86899759edaafafcc
• Download URL:
  https://stacks.cdc.gov/view/cdc/192760/cdc_192760_DS1.pdf
• File Type:
  [PDF - 559.85 KB ]