Validation of an HPLC-MS-MS method for the determination of urinary s-benzylmercapturic acid and s-phenylmercapturic acid

Details

• Personal Author:
  B'Hymer, Clayton
• Description:
  A high-performance liquid chromatography-tandem mass spectrometric (HPLC-MS-MS) method is presented and evaluated for the determination of S-benzylmercapturic acid (S-BMA) and S-phenylmercapturic acid (S-PMA) in human urine. Both of these compounds are important for occupational health owing to their use as biomarkers of exposure to toluene and benzene, respectively. Toluene is used extensively as a solvent, and the health hazards of benzene have been well established. The optimized urine sample preparation scheme consists of solid-phase extraction (SPE) followed by an acetone wash. The chromatographic analysis consists of a reversed-phase gradient system, which uses electrospray ionization in negative-ion mode with a triple-quadrupole mass spectrometric detector. Accuracy and precision of this method are demonstrated by a series of recovery studies of spiked human urine and synthetic urine substitute. Spike levels at 1, 2, 6, 8, and 30 ng/mL for both analytes demonstrate average recoveries (accuracy) ranging from 99 to 110 percent. Precision as measured by the relative standard deviation (percentRSD) of multiple samples (n = 9) at each concentration level was 5.3 percent or less for both analytes in urine. The limit of detection (LOD) is approximately 0.2 ng/mL for S-BMA and S-PMA. This data, other figures of merit and other factors, such as ion suppression of the electrospray ionization source, are discussed. [Description provided by NIOSH]
• Subjects:
  Acids Benzene Biomarkers Energy Metabolism Laboratories Mass Spectrometry Occupational Health Safety Solvents Sulfhydryl Compounds Toluene Urine

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• Keywords:
  Analytical-instruments Analytical-processes Benzenes Electrochemical-analysis Ionization Laboratory-techniques Liquid-chromatography Mass-spectrometry Mercaptans Metabolites Phenyls Sample-preparation Sampling Sampling-equipment Sampling-methods Toluenes Urinalysis Urine-chemistry

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• ISSN:
  0021-9665
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  49
• Issue:
  7
• NIOSHTIC Number:
  nn:20039233
• Citation:
  J Chromatogr Sci 2011 Aug; 49(7):547-553
• Contact Point Address:
  Clayton B'Hymer, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, Mail Stop C-23, 4676 Columbia Parkway, Cincinnati, OH 45226, USA
• Email:
  cbhymer@cdc.gov
• CAS Registry Number:
  Benzene (CAS RN 71-43-2) ; Toluene (CAS RN 108-88-3)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Healthcare and Social Assistance ; Services ; Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Chromatographic Science
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:2ce0eae23ebae94b67c2b7a092db2e7d8fbe36229d0bbb6e258be778871537b3212457632ae9511a2380cdce9fc389ff5f98e17fc26a7489df1dc978935b313b
• Download URL:
  https://stacks.cdc.gov/view/cdc/192649/cdc_192649_DS1.pdf
• File Type:
  [PDF - 1.59 MB ]