Identification of systemic markers from a pulmonary carbon nanotube exposure

Details

• Personal Author:
  Castranova, Vincent ; Erdely A ; Hulderman T ; Liston AL ; Salmen-Muniz R ; Simeonova PP ; Young SH ; Zeidler-Erdely PC
• Description:
  Currently there is expanding interest, from the perspectives of occupational health surveillance and future epidemiological research, in early monitoring of worker exposure to engineered nanomaterials. Here, we highlight quantitative systemic markers in mice after a single exposure to carbon nanotubes (CNT) (multi-walled (MW) CNT or single-walled (SW) CNT). Mice were exposed by pharyngeal aspiration to 40microg and harvested at 24 hr, 7 and 28d post exposure. Mice were sacrificed and the following parameters were analyzed: whole blood gene expression, blood and bronchoalveolar lavage (BAL) differentials, serum protein profiling by immunoplex and proteomics, and gene expression analysis in the lung and extrapulmonary tissues including aorta, heart and liver. Early effects of pulmonary CNT exposure include a rapid but transient increase in inflammatory blood gene expression (IL-1beta, CXCL2) and serum cytokines (IL-6, IL-5, CCL11). This was followed by an acute phase response including CRP, SAA-1, SAP and haptoglobin as shown by elevated serum protein levels and liver gene expression. Beyond 24hr there was a consistent increase in both blood and BAL eosinophils. These data correspond well with early increased serum IL-5 and CCL11 after MWCNT exposure. At 28d, serum acute phase proteins with immune function including complement C3, apolipoprotein A-I and A-II, alpha2-marcoglobulin, serotransferrin and liver carboxylesterase N were increased. The systemic markers correlated well with existing literature showing end-point measurements of pulmonary CNT exposure and adverse cardiovascular effects and enhanced allergic response. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Cardiovascular Diseases Cardiovascular System Epidemiology Genetics Hypersensitivity Irritants Laboratories Lung Lung Diseases Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Airborne-particles Allergic-reactions Biological-effects Cardiopulmonary-system Cardiopulmonary-system-disorders Cardiovascular-disease Cardiovascular-system Cardiovascular-system-disorders Cellular-reactions Exposure-levels Genetic-factors Inhalation-studies Laboratory-animals Lung-disorders Lung-irritants Lung-tissue Nanotechnology Physiological-effects Pulmonary-disorders Pulmonary-system Pulmonary-system-disorders Quantitative-analysis Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  120
• NIOSHTIC Number:
  nn:20038599
• Citation:
  Toxicologist 2011 Mar; 120(Suppl 2):320
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2011
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC
• Supplement:
  2
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:28d306b32fd8d66f37198229da5bc22f0e05e22ec9cbfdee1622592e1d02d933c878c7022ba8aa10fa63eb86a7aed5e48b3709ac6b7069c4d8458df4cf288f9e
• Download URL:
  https://stacks.cdc.gov/view/cdc/192251/cdc_192251_DS1.pdf
• File Type:
  [PDF - 1.10 MB ]