Nanoparticle inhalation enhances cardiac protein phosphorylation and neurotransmitter synthesis in the nodose ganglia of rats

Details

• Personal Author:
  Castranova, Vincent ; Chen F ; Chen TB ; Cumpston JL ; Kan H ; Wu Z ; Young S ; Castranova, Vincent ; Chen F ; Chen TB ; Cumpston JL ; Kan H ; Wu Z ; Young S
• Description:
  Growing evidence from epidemiological studies indicates that an increase of the small sized particle, particularly nanoparticle, component in ambient air is strongly associated with increased incidence of cardiovascular diseases. Animal studies demonstrated that pulmonary inhalation of nanoparticles stimulates the development of atherosclerosis and impairs vascular function. However, the effect of inhaled nanoparticles on cardiac muscle has not been reported. The present study investigated the effect of ultrafine titanium dioxide (UFTiO2) on the heart. We found that direct exposure of rat cardiac myocytes in vitro to UFTiO2 (1 mu g/ml) for 4 hrs did not alter myocyte contractility, calcium handling, or the phosphorylation level of cardiac proteins, such as p38 mitogen-activated protein kinase (p38 MAPK) and cardiac troponin I (cTnI). In contrast, pulmonary inhalation of UFTiO2 (6 mg/m3) for 4 hrs significantly increased the phosphorylation status of p38 MAPK and cardiac cTnI in the heart. In addition, pulmonary exposure to UFTiO2 also increased neurotransmitter substance P synthesis in nodose ganglia, which is involved in the integration and control of lung and heart function. However, neither mRNA expression nor protein synthesis of TNF-alpha, IL-1 and IL- 6 was detected in the heart or peripheral blood respectively. Blood cell counts and differentials did not indicate significant systemic inflammation. Our results suggested that pulmonary exposure to UFTiO2-enhanced the phosphorylation level of p38 MAPK and cTnI in cardiac tissue. Such responses may contribute to cardiac dysfunction which is independent of the direct interaction of UFTiO2 with the heart or systemic inflammation, but may involve a lung-neuron-regulated pathway. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Cardiovascular Diseases Cardiovascular System Epidemiology Hazardous Substances Irritants Laboratories Lung Lung Diseases Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Airborne-particles Biohazards Biological-effects Cardiopulmonary-system Cardiopulmonary-system-disorders Cardiovascular-disease Cardiovascular-system Cardiovascular-system-disease Cardiovascular-system-disorders Cellular-reactions Exposure-levels Inhalation-studies Laboratory-animals Lung-disorders Lung-irritants Nanotechnology Particulates Physiological-effects Pulmonary-disorders Pulmonary-system Pulmonary-system-disorders Quantitative-analysis Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders Statistical-analysis

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Abstract or Summary
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  120
• NIOSHTIC Number:
  nn:20038590
• Citation:
  Toxicologist 2011 Mar; 120(Suppl 2):313
• CAS Registry Number:
  Titania (CAS RN 13463-67-7) ; Titania (CAS RN 13463-67-7)
• Federal Fiscal Year:
  2011
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC
• Supplement:
  2
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:f13f4a6371cb3b416f468347a3111cad7a44aff4de951ec335dc31d6f51de08c5f64667742933f8c32c7f1823f588667d61c9f5ca097097b4f1f1656113cfa21
• Download URL:
  https://stacks.cdc.gov/view/cdc/192243/cdc_192243_DS1.pdf
• File Type:
  [PDF - 1.10 MB ]