Zone-specific changes in micromechanical, biochemical, and structural properties in articular cartilage from a rabbit joint flexion model

Details

• Personal Author:
  King KB ; Li C ; Pruitt LA
• Description:
  Metacarpophalangeal (MCP) joint proximal bone-cartilage specimens from the fourth digit were collected from repetitively flexed and non-flexed (control) paws of four New Zealand White rabbits. The specimens were cryo-fractured to reveal a sagittal cut containing the cartilage zones of different collagen microstructure. Nanoindentation, Fourier Transform infrared microspectroscopic imaging (FTIRMI), and histology were performed on a region of interest (ROI) approximately 400 microns wide and through the thickness of the cartilage with two goals in mind: (1) to examine the effect of collagen network structure (random in the mid zone versus organized in the deep zone) on the biomechanical and biochemical properties of cartilage; and (2) to understand the changes in these properties due to physical forces. We found that zone microstructure significantly affected the measurement of the local relaxed modulus measured by nanoindentation. The deep zone had a higher modulus than the mid zone (Wilcoxon paired test, p<0.05). We also found that flexion significantly decreased the proteoglycan content in both the mid and deep zones (Wilcoxon paired test, p<0.05), suggesting indirect repetitive loading in the rabbit paw can be damaging to the joint via down regulating proteoglycan synthesis in the mid and deep zone cartilage. This is the first study to simultaneously report the local zone-specific mechanical and biochemical properties in the rabbit joint flexion model. [Description provided by NIOSH]
• Subjects:
  Animals Biomechanical Phenomena Cumulative Trauma Disorders Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Animal-studies Author Keywords: Tissue Biomechanics Cumulative-trauma Cumulative-trauma-disorders Elastic Properties Hand-injuries Laboratory-animals Muscles Musculoskeletal-system-disorders Repetitive-work Structural

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• ISSN:
  0272-9172
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; Colorado ; OSHA Region 8 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-8
• Volume:
  1097
• NIOSHTIC Number:
  nn:20038530
• Citation:
  Mater Res Soc Symp Proc 2008 Apr; 1097(GG):1-8
• Email:
  cli@newton.berkeley.edu
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
• Performing Organization:
  University of California - San Francisco
• Peer Reviewed:
  False
• Start Date:
  20010701
• Source Full Name:
  Materials Research Society Symposia Proceedings
• End Date:
  20060630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:0cdc5da58092d2fd60e44ea77226f40407b1e4f5353aa2437e964dd559298330f7d31ee5d56883e3d8da75617e3023db47fdcb481b87f0a3163f321e5ffb0c0e
• Download URL:
  https://stacks.cdc.gov/view/cdc/192209/cdc_192209_DS1.pdf
• File Type:
  [PDF - 490.83 KB ]