Nanoindentation of finger joint cartilage in a fluid cell

Details

• Personal Author:
  Ebenstein DM ; King K ; Li C ; Pruitt LA
• Description:
  Assessing the mechanical properties of diarthrodial joint cartilage is important for the treatment of osteoarthritis and the development of functional tissue-engineered cartilage. The traditional techniques of confined and unconfined compression performed on cartilage plugs and more recent in-situ indentation measurements have shown articular cartilage mechanical properties to be site, depth, and direction-dependent. Further these techniques are limited to larger specimens with relatively smooth joint surfaces. In a preliminary study, nanoindentation performed on rabbit finger joint cartilage has been shown effective in detecting local mechanical properties. Nanoindentation can target the large variety of positions and topographies found in small areas allowing direct assessment of structure-function relationships at the tissue-scale (10-4-10-2 m) and micro-scale (10-7-10-4 m) levels. Such information can provide critical insight into the mechanisms of cartilage function in load support and lubrication, as well as joint degeneration. This study aims to 1) validate the modified nanoindentation technique for mechanical measurements of articular cartilage in a fluid environment and 2) evaluate hydration as a critical testing parameter. In-situ compressive reduced modulus was measured for healthy rabbit metacarpophalangeal (MCP) joint cartilage immersed in saline with a specialized fluid cell tip at fine spatial resolution. [Description provided by NIOSH]
• Subjects:
  Animals Biomechanical Phenomena Cumulative Trauma Disorders Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Animal-studies Biomechanics Cumulative-trauma Cumulative-trauma-disorders Hand-injuries Laboratory-animals Muscles Musculoskeletal-system-disorders Repetitive-work
• ISSN:
  0149-6433
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Proceedings
• Place as Subject:
  California ; Colorado ; OSHA Region 8 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  50
• NIOSHTIC Number:
  nn:20038558
• Citation:
  Trans Annu Meet Orthop Res Soc 2004 Mar; 50:0539
• Federal Fiscal Year:
  2004
• NORA Priority Area:
  Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
• Performing Organization:
  University of California - San Francisco
• Peer Reviewed:
  False
• Start Date:
  20010701
• Source Full Name:
  Transactions of the Annual Meeting of the Orthopaedic Research Society
• End Date:
  20060630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7ea3afe64f93041ae284d73fc920a8ff264d38b6f7e8f2c27a3b5978ee4f42087b8be662b5f6ef38b0cc82b6f402bdae261bd94c659779797197281a49d76906
• Download URL:
  https://stacks.cdc.gov/view/cdc/192218/cdc_192218_DS1.pdf
• File Type:
  [PDF - 89.95 KB ]