Pulmonary fibrotic response to subchronic multi-walled carbon nanotube exposure [2011-03]

Details

• Personal Author:
  Battelli LA ; Castranova, Vincent ; Hubbs, Ann F. ; Mercer RR ; Porter DW ; Scabilloni JF ; Wang L
• Description:
  Multi-walled carbon nanotubes (MWCNTs) are manufactured carbon compounds with many commercial applications. To address the hypothesis that MWCNTs cause persistent pulmonary pathology, C57BL/6J mice were exposed by pharyngeal aspiration to 10, 20, 40 or 80 ug MWCNTs (mean dimensions of 3.9 um x 49 nm) or vehicle. Lungs were preserved at 1, 7, 28 and 56 days post exposure to analyze the distribution of lung burden. Morphometric measurement of Sirius Red staining was used to assess the connective tissue response. At day 1 post-exposure 62.0+/-2.5 and 9.9+/-2 percent of the lung burden (mean+/-SE, N=7) were in alveolar macrophages and alveolar tissue, respectively. The remainder of the lung burden (18.0+/-3.2) was in the airways. By 56 days post-exposure, 68.7+/-3.9, 7.5+/-1.9 and 22.0+/-5.1 percent of MWCNT were in alveolar macrophages, alveolar tissue and granulomatous lesions, respectively. No MWCNTs were found in the airways at 56 days. At 56 days post-exposure the average thickness of connective tissue in alveolar regions was 0.11+/-0.01, 0.12+/-.01, 0.12 +/-0.01, 0.16+/-0.01 and 0.19+/-0.01 um (mean+/-SE, N=6) for vehicle, 10, 20, 40 and 80 ug dose groups, respectively. The connective tissue in the alveolar region demonstrated a progressive increase in thickness over time in the 80 ug exposure group (0.11+/-0.01, 0.14 +/-0.01, 0.16+/-0.01 and 0.19+/-0.01 um for 1, 7, 28 and 56 day). The distribution of lung burden was predominately within alveolar macrophages with approximately 8% delivery to the alveolar tissue. Despite the relatively low fraction of the lung burden being delivered to the alveolar tissue (7.5% at day 56), the average thickness of connective tissue in the alveolar region was increased over vehicle control by 45% in the 40 ug and 72% in 80 ug exposure groups. These results demonstrate that MWCNT have the potential to produce a progressive, fibrotic response in the alveolar tissues of the lungs. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution, Indoor Cytotoxins Hazardous Substances Irritants Lung Lung Diseases Microbiology Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety

  More +
• Keywords:
  Air-contamination Airborne-particles Biological-effects Cell-biology Cellular-reactions Cytotoxicity Dose-response Exposure-levels Inhalation-studies Lung-disorders Lung-fibrosis Lung-irritants Microscopic-analysis Nanotechnology Physiological-effects Pulmonary-system-disorders Quantitative-analysis Respiratory-hypersensitivity Respiratory-irritants Respiratory-system-disorders Statistical-analysis Toxic-effects

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  11
• Volume:
  120
• NIOSHTIC Number:
  nn:20038436
• Citation:
  Toxicologist 2011 Mar; 120(Suppl 2):11
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0)
• Federal Fiscal Year:
  2011
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 50th Annual Meeting and ToxExpo, March 6-10, 2011, Washington, DC
• Supplement:
  2
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9022a577223bb2efb8f9a84d85b1825160feb4fb0e01ac393aef90a48732e2d4cd46950bc08f71b63dfb785cd4656b81bb78100c12a9f5188115178fc2dcb5be
• Download URL:
  https://stacks.cdc.gov/view/cdc/192149/cdc_192149_DS1.pdf
• File Type:
  [PDF - 1.09 MB ]