Quantitative plasma biomarker analysis in HDI exposure assessment

Details

• Personal Author:
  Ball LM ; Fent, Kenneth W. ; Flack SL ; Nylander-French LA ; Thomasen JM ; Trelles Gaines LG ; Wittaker S
• Description:
  Quantification of amines in biological samples is important for evaluating occupational exposure to diisocyanates. In this study, we describe the quantification of 1,6-hexamethylene diamine (HDA) levels in hydrolyzed plasma of 46 spray painters applying 1,6-hexamethylene diisocyanate (HDI)-containing paint in vehicle repair shops collected during repeated visits to their workplace and their relationship with dermal and inhalation exposure to HDI monomer. HDA was detected in 76% of plasma samples, as heptafluorobutyryl derivatives, and the range of HDA concentrations was < or =0.02-0.92 microg l(-1). After log-transformation of the data, the correlation between plasma HDA levels and HDI inhalation exposure measured on the same workday was low (N = 108, r = 0.22, P = 0.026) compared with the correlation between plasma HDA levels and inhalation exposure occurring approximately 20 to 60 days before blood collection (N = 29, r = 0.57, P = 0.0014). The correlation between plasma HDA levels and HDI dermal exposure measured on the same workday, although statistically significant, was low (N = 108, r = 0.22, P = 0.040) while the correlation between HDA and dermal exposure occurring approximately 20 to 60 days before blood collection was slightly improved (N = 29, r = 0.36, P = 0.053). We evaluated various workplace factors and controls (i.e. location, personal protective equipment use and paint booth type) as modifiers of plasma HDA levels. Workers using a downdraft-ventilated booth had significantly lower plasma HDA levels relative to semi-downdraft and crossdraft booth types (P = 0.0108); this trend was comparable to HDI inhalation and dermal exposure levels stratified by booth type. These findings indicate that HDA concentration in hydrolyzed plasma may be used as a biomarker of cumulative inhalation and dermal exposure to HDI and for investigating the effectiveness of exposure controls in the workplace. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Biomarkers Environmental Monitoring Inhalation Exposure Irritants Lung Occupational Health Paint Particulate Matter Respiratory Hypersensitivity Respiratory System Safety Workplace

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• Keywords:
  1,6-hexamethylene Diamine (HDA) 1,6-hexamethylene Diisocyanate (HDI) Airborne-particles Author Keywords: Biomarker Biological-effects Biological-systems Breathing Breathing-zone Dermal Exposure Exposure-assessment Exposure-levels Inhalants Inhalation Exposure Lung-irritants Paint-shops Paint-spraying Painters Particle-aerodynamics Particulates Plasma Pulmonary-system Quantitative-analysis Respiratory-hypersensitivity Respiratory-irritants Spray-painting Statistical-analysis Workplace-studies

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• ISSN:
  0003-4878
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  41-54
• Volume:
  54
• Issue:
  1
• NIOSHTIC Number:
  nn:20038318
• Citation:
  Ann Occup Hyg 2010 Jan; 54(1):41-54
• Contact Point Address:
  Leena A. Nylander-French, Department of Environmental Sciences and Engineering, Gillings School of Global Public Health, The University of North Carolina at Chapel Hill, CB #7431, Rosenau Hall, Chapel Hill, NC 27599-7431
• Email:
  leena_french@unc.edu
• CAS Registry Number:
  Hexamethylene diisocyanate (CAS RN 822-06-0)
• Federal Fiscal Year:
  2010
• Performing Organization:
  University of North Carolina, Chapel Hill, North Carolina
• Peer Reviewed:
  True
• Start Date:
  20030801
• Source Full Name:
  Annals of Occupational Hygiene
• End Date:
  20070731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:acb79e831805051f6838a039d4a7b1072bd5e7af437e2b452e26b6940c6b1dabc9c544cf6b3acd1339943d5090ae8dcef50662b39a634d54c24d89e18a0c7ab5
• Download URL:
  https://stacks.cdc.gov/view/cdc/192065/cdc_192065_DS1.pdf
• File Type:
  [PDF - 245.06 KB ]