A rapid HPLC analysis for dermal penetration: the case study of 4-chloro-3-methylphenol (CMP) from metal working fluid Trim VX

Details

• Personal Author:
  Barbero AM ; Frasch, H. Fredrick ; Zang L-Y
• Description:
  A rapid and simple HPLC method has been developed for the determination of 4-chloro-3-methylphenol (CMP) in the metal working fluid (MWF), Trim VX and in experiments designed to measure the dermal penetration of CMP from MWF. The method used 2-methyl-4,6-dinitrophenol as an internal standard (IS). Water was used as the extraction solvent. The retention times of CMP and internal standard (IS) were 5.56 and 6.67 min, respectively. The standard plots of CMP are linear over the range of 62.5 - 2,500 ng·mL-1 with correlation coefficients of 0.999. The limit of quantification (LOQ) for CMP was estimated to be 62.5 ng·mL-1 and the limit of detection (LOD) was approached to be 20 ng·mL-1. Reproducibility of the sample handling and HPLC analysis had a relative standard deviation (RSD) of less than 6%. The average recoveries for the analyte in Trim VX were better than 92%. Using the method, we found that Trim VX contains 2% CMP. In our preliminary dermal penetration study, the permeability coefficient kp and lag time (tau) were estimated to be 4.88..10-3 cm·h-1 and 1.65 h, respectively. [Description provided by NIOSH]
• Subjects:
  Metals Metal Workers Occupational Health Safety Skin Skin Absorption
• Keywords:
  Analytical-methods Analytical-processes Author Keywords: CMP HPLC Liquid-chromatography Metal-compounds Metalworking Metalworking-fluids MWFs Skin-absorption Skin-exposure Skin-irritants

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• ISSN:
  1874-0650
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  10-17
• Volume:
  4
• NIOSHTIC Number:
  nn:20037939
• Citation:
  Open Anal Chem J 2010 Mar; 4:10-17
• Contact Point Address:
  H. Frederick Frasch, Exposure Assessment Branch, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, Morgantown, WV 26505-2888, USA
• Email:
  hbf9@cdc.gov
• CAS Registry Number:
  4-Chloro-3-methylphenol (CAS RN 59-50-7)
• Federal Fiscal Year:
  2010
• Peer Reviewed:
  False
• Source Full Name:
  The Open Analytical Chemistry Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:896325fc28e5484a594a904f8592447e955b75a5a1549e00fa6fa452222e579298187417590c7478abd0f5b7052c72aea6aab549d136f756c11c33b6aca591bf
• Download URL:
  https://stacks.cdc.gov/view/cdc/191846/cdc_191846_DS1.pdf
• File Type:
  [PDF - 1.11 MB ]