Nanoindentation Differentiates Tissue-Scale Functional Properties of Native Articular Cartilage

Details

• Personal Author:
  King KB ; Li C ; Pruitt LA
• Description:
  Cartilage mechanical properties are typically tested at the macroscale. To demonstrate the ability of nanoindentation to characterize in situ articular cartilage properties at the tissue scale, we investigated the local structure-property relationships of intact articular cartilage of a normal rabbit metacarpophalangeal joint. We calculated the mechanical parameters of stiffness, S, resistance to penetration, R, and volumetric creep strain, dV/V, from nanoindentation of the articular surface at specific regions of interest. We measured morphological parameters of superficial zone thickness, middle zone thickness, total uncalcified thickness, and cell density from corresponding regions with light and polarized light microscopy. Mechanical parameters were compared to morphological parameters. There were significant positive correlations (r = 0.98, p < 0.05) between superficial zone thickness and both S and R. However, we found no significant correlation between dV/V and the zone sizes. There were moderate, negative correlations between cell density and both S and R, suggesting an effect of cell volume on cartilage behavior at the tissue scale. We opine that the superficial zone plays important role in load support, as evidenced by correlations between zone size and intact cartilage mechanical properties. [Description provided by NIOSH]
• Subjects:
  Animals Biomechanical Phenomena Cells, Cultured Cytology Laboratories Occupational Health Safety
• Keywords:
  Animal-studies Biomechanics Cell-cultures Cell-differentiation Laboratory-animals
• ISSN:
  1549-3296
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  California ; Colorado ; OSHA Region 8 ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  78
• Issue:
  4
• NIOSHTIC Number:
  nn:20031154
• Citation:
  J Biomed Mater Res A 2006 Sep; 78(4):729-738
• Email:
  Karen.King@ucsf.edu
• Federal Fiscal Year:
  2006
• NORA Priority Area:
  Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
• Performing Organization:
  Northern California Educaton and Research Center, Center for Occupational and Environmental Health, University of California, School of Public Health, Berkeley, California
• Peer Reviewed:
  True
• Start Date:
  19970701
• Source Full Name:
  Journal of Biomedical Materials Research. Part A
• End Date:
  20020630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:17243b8e56b57061b4ec9c1aed6a10e3a14a1708a5e29a3d1133539f8fffb257a761cccb0b3c2598b9119bc52dd8d4d9d607d856e5e2d077fa9bd193ebacbee7
• Download URL:
  https://stacks.cdc.gov/view/cdc/191490/cdc_191490_DS1.pdf
• File Type:
  [PDF - 616.38 KB ]