Glucose Partition Coefficient and Diffusivity in the Lower Skin Layers

Details

• Personal Author:
  Kasting GB ; Khalil E ; Kretsos K
• Description:
  PURPOSE: This work aims to estimate the diffusivity and partitioning of glucose in the dermis and the viable epidermis of human skin. METHODS: The partition coefficient of glucose between phosphate-buffered saline and dermis, tape-stripped epidermis (TSE), stratum corneum (SC), and split-thickness skin, was measured in vitro using human cadaver skin. Glucose permeability across dermis and tape-stripped split-thickness skin (TSS) was measured using side-by-side diffusion cells. Glucose desorption from TSE and human epidermal membrane (HEM) was measured. All measurements were conducted at 32 degrees C. RESULTS: The partition coefficient for glucose [mean +/- SD (no. of samples)] was 0.65 +/- 0.09 (n = 25) for dermis, 0.81 +/- 0.06 (n = 10) for TSE, and 0.53 +/- 0.12 (n = 9) for SC. Glucose diffusivity in dermis was calculated to be 2.64 +/- 0.42 x 10(-6) cm2/s (n = 14). Glucose diffusivities in the viable epidermis estimated from TSS permeation, TSE desorption, and HEM desorption were 0.075 +/- 0.050 x 10(-6) cm2/s (n = 5), 0.037 +/- 0.018 x 10(-6) cm2/s (n = 4), and 1.0 +/- 0.6 x 10(-6) cm2/s (n = 4), respectively. CONCLUSION: The tissue/buffer partition coefficient of glucose in all skin layers was found to be less than unity, suggestive of excluded volumes in each layer. Glucose diffusivity in human dermis was found to be one third of its value in water, indicative of hindered diffusion related to the structural components of the tissue. A substantially lower value for glucose diffusivity in viable epidermis is suggested. [Description provided by NIOSH]
• Subjects:
  Animals Cytology Laboratories Occupational Health Safety Skin
• Keywords:
  Animal-studies Dermatology Diffusion-analysis Humans In-vitro-studies In-vitro-study Laboratory-animals Skin-tests
• ISSN:
  0724-8741
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  23
• Issue:
  6
• NIOSHTIC Number:
  nn:20031615
• Citation:
  Pharm Res 2006 Jun; 23(6):1227-1234
• Contact Point Address:
  Faculty of Pharmacy, The University of Jordan, Amman, 11942, Jordan
• Email:
  ekayoub@ju.edu.jo
• Federal Fiscal Year:
  2006
• NORA Priority Area:
  Research Tools and Approaches: Exposure Assessment Methods
• Performing Organization:
  University of Cincinnati
• Peer Reviewed:
  True
• Start Date:
  20020901
• Source Full Name:
  Pharmaceutical Research
• End Date:
  20130731
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:102e8cb5e52f1d5ee51b1ae75c1a1c26eba90e56283d3845f349a394a9e2a5c302adfee3c182376d7ca90a64905b775e7ff96f27689108cb00d40985f515c3a7
• Download URL:
  https://stacks.cdc.gov/view/cdc/185860/cdc_185860_DS1.pdf
• File Type:
  [PDF - 324.23 KB ]