Survanta Does Not Inhibit Nitric Oxide (NO) Production Induced by Lipopolysaccharide (LPS) and Interferon-y in Two Macrophage Cell Lines

Details

• Personal Author:
  Bowman L ; Meighan T ; Rao KMK
• Description:
  We have previously shown that lung surfactant and Survanta(a surfactant substitute) inhibit NO production induced by LPS in rat alveolar macrophages (Miles et al., Am.J.Physiol., in press). We studied the effect of Survanta (200 microg phospholipid/ml) on NO production in two monocytic cell lines, J-774A.l and Raw-264.7, and found that it does not inhibit NO production induced by LPS (1 microg/ml) plus interferon-y (25 U/ml). The effect on NO was characterized by measuring NO production by the Griess reaction, NO message by a ribonuclease protection assay and NO protein synthesis by Western blotting. Survanta had no effect on any of the parameters. In contrast, interleukin-13 (IL-I3), a cytokine known to inhibit NO production by decreasing NO message and protein synthesis, had the expected effect in these two cell lines. The observations indicate that the effect of lung surfactant on NO production is cell-specific. In alveolar macrophages, lung surfactant decreases NO production by decreasing NO protein levels without decreasing NO message levels, indicating that the regulation may be occurring at the transitional level. The differential effects of Survanta on rat alveolar macrophages and these monocytic cell lines may offer a useful model for delineating the molecular mechanisms, regulating NO production in inflammatory cells. [Description provided by NIOSH]
• Subjects:
  Animals Chemistry Chemistry, Analytic Environmental Monitoring Hazardous Substances Irritants Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety Surface-Active Agents Toxicology

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• Keywords:
  Alveolar-cells Analytical-chemistry Analytical-methods Animal-studies Exposure-assessment Laboratory-animals Pulmonary-disorders Pulmonary-system-disorders Respiratory-irritants Respiratory-system-disorders Statistical-analysis Surfactants Toxic-materials Toxins

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• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  District of Columbia ; OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  13
• Issue:
  4
• NIOSHTIC Number:
  nn:20030697
• Citation:
  FASEB J 1999 Mar; 13(4)(Pt 2):A1150
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  1999
• Peer Reviewed:
  False
• Part Number:
  2
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6dbb89ed4512c01fa8b8b276f0822b15d7c505e5059e5eadb9edf99a0d19d3da7d15a4b2a793de6dd19e199c3c2c93fa23a77551344c8a6f445a14797b1e143d
• Download URL:
  https://stacks.cdc.gov/view/cdc/191225/cdc_191225_DS1.pdf
• File Type:
  [PDF - 419.42 KB ]