Effects of Na+ and Cl- Channel Blockers on Guinea-Pig (GP) Isolated Tracheal Epithelial Cell (EC) Shrinkage in Response to Hyperosmolar Challenge

Details

• Personal Author:
  Dowdy JA ; Fedan, Jeffery S. ; Ismailoglu UB ; Jing Y ; Van Scott MR
• Description:
  In GP trachea that are precontracted with methacholine (MCh; 3H10'^-7 M), hyperosmolar D-mannitol (D-M) induces changes in epithelial Na+ and Clr transport and smooth muscle relaxation mediated by epitheliumderived relaxing factor (EpDRF). We examined the effects of sequentially-added ion channel blockers, MCh and D-M (30 mosM) on EC cell volume (CV) changes in ECs isolated enzymatically (2% protease, 1 h) from GP-trachea and suspended in Krebs-Henselejt solution, under conditions in which these agents are used in the isolated, perfused trachea preparation to examine D-M-induced EpDRF release. CV was measured with a cell sizer. D-M elicited shrinkage of EC (approximately 15 %) under conditions in which lit caused EpDRF-mediated relaxation. The Na+ channel blocker, amiloride (A; 10^-4 M), did not affect EC CV. MCh had no effect on CV (+/- A). Shrinkage stimulated by D-M was unaffected by A. On the other hand, incubation with the clr blocker, DIDS (10^-4 M), resulted in an immediate and prolonged decrease in CV (approximately 9%). Again, MCh had no effect on CV (+/- DIDS). However, in the presence of DIDS the CV decrease in response to D-M was diminished. Thus, the inhibitory effect of A on EpDRF release is not due to an effect on EC shrinkage, whereas the inhibitory effect of DIDS on EpDRF release may be linked to the decrease in, the CV response. [Description provided by NIOSH]
• Subjects:
  Animals Cells, Cultured Laboratories Occupational Health Safety
• Keywords:
  Animal-studies Cell-biology Cell-cultures Laboratory-animals
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  20
• Issue:
  5
• NIOSHTIC Number:
  nn:20030031
• Citation:
  FASEB J 2006 Mar; 20(5)(Pt 2):A835
• Federal Fiscal Year:
  2006
• Peer Reviewed:
  False
• Part Number:
  2
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:bf18de40c8260705e9593aeab7eb97fcfbead7a27dcdec22a86b0986ed1b8caf98aff3cbfe434d18ddc41b0120977500302c6e3e237c24b65c0d2e4382b3dc8e
• Download URL:
  https://stacks.cdc.gov/view/cdc/190887/cdc_190887_DS1.pdf
• File Type:
  [PDF - 436.60 KB ]