Guinea-Pig Tracheal Epithelial Cell (EC) Shrinkage Induced by Hyperosmolar Challenge

Details

• Personal Author:
  Dowdy JA ; Fedan, Jeffery S. ; Ismailoglu UB ; Jing Y ; Van Scott MR
• Description:
  Hyperventilation during exercise is thought to increase the osmolarity of airway surface liquid. Hyperosmolar challenge of EC with mucosal Dmannitol (D-M) in methacholine (MCh)-contracted guinea-pig isolated, perfused trachea elicits smooth muscle relaxation mediated by epithelium-derived relaxing factor (EpDRF). To understand the relationship between EpDRF release and hyperosmolarity-induced changes in cell volume (CV), we examined CV changes in response to challenge of EC with 120 mosM D-M. EC were isolated from tracheas by protease digestion (2%; 1 h) and suspended in Krebs-Henseleit solution. Suspended EC remained viable for more than 5 h as indicated by active beating of cilia and exclusion of trypan blue (90 to 95%). CV was measured using a cell sizer. D-M induced a rapid decrease in CV ( approximately 9% shrinkage) during the first minute. The peak CV decrease occurred between 3 to 5 min (approximately 22% shrinkage). No increase in CV was observed during the next 5 h. The results indicate that hyperosmolar conditions which cause EpDRF-mediated relaxation also evoke EC shrinkage. The rapid onset of the relaxation and CV responses could suggest that signaling pathways are shared. [Description provided by NIOSH]
• Subjects:
  Animals Cells, Cultured Environmental Monitoring Laboratories Occupational Health Safety
• Keywords:
  Animal-studies Cell-biology Cell-cultures Exposure-assessment Exposure-levels Laboratory-animals Surface-properties
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  North Carolina ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  20
• Issue:
  5
• NIOSHTIC Number:
  nn:20030030
• Citation:
  FASEB J 2006 Mar; 20(5)(Pt 2):A835
• Federal Fiscal Year:
  2006
• Peer Reviewed:
  False
• Part Number:
  2
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:39c318210c7e0a16f61858c340b206a6946008032ca4483e9189df224e73a9524ba0144260cc85cc8aebdd2e7c2e124ee727d0f115b6ec556e7c58d41db14dd6
• Download URL:
  https://stacks.cdc.gov/view/cdc/190886/cdc_190886_DS1.pdf
• File Type:
  [PDF - 441.50 KB ]