Attenuation of Stretch-Induced Histopathologic Changes of Skeletal Muscles by Quinacrine

Details

• Personal Author:
  Stauber WT ; Willems ME
• Description:
  Quinacrine is an inhibitor of phospholipase A(2), an enzyme thought to be involved in activity-related injury of skeletal muscles. Histopathologic changes after injury by stretches of activated plantar-flexor muscles were measured in untreated and quinacrine-treated rats. On day 4 of treatment (50 mg.kg(-1) intraperitoneally for 5 days), 30 stretches were induced by ankle rotation after muscles reached a maximal isometric force. During the stretch protocol, peak stretch forces and isometric force deficits after each stretch [total deficits 56.7 +/- 2.8% (untreated rats) and 59.6 +/- 1.7% (quinacrine-treated rats)] were similar for both groups (n = 6 each). Two days after the stretch protocol, histopathologic changes were evaluated using antibody staining on cross-sections of gastrocnemius medialis muscles. Swollen myofibers devoid of desmin were identified. Similar cells, but not all swollen myofibers, in adjacent sections stained for albumin. Quinacrine reduced the number of desmin-negative and albumin-positive cells by 88% (P < 0.05) and 84% (P < 0.05), indicating that it attenuated histopathologic changes that follow stretch injury of activated skeletal muscles. Histopathologic changes following muscle injury or myopathic disease may thus be reduced or even prevented by selective drug intervention, thereby reducing the risk of muscle fibrosis. [Description provided by NIOSH]
• Subjects:
  Animals Enzymes Fibrosis Laboratories Musculoskeletal Diseases Musculoskeletal System Occupational Health Safety
• Keywords:
  Animal Studies Drugs Enzyme Inhibitors Histopathology Injuries Injury Prevention Laboratory Animals Muscle Contraction Muscles Musculoskeletal System Disorders Skeletal System Disorders

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• ISSN:
  0148-639X
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  65-71
• Volume:
  27
• Issue:
  1
• NIOSHTIC Number:
  nn:20029272
• Citation:
  Muscle Nerve 2003 Jan; 27(1):65-71
• Contact Point Address:
  Department of Physiology and Pharmacology, West Virginia University, P.O. Box 9229, Morgantown, West Virginia 26506-9229, USA
• Email:
  mwillems@hsc.wvu.edu
• Federal Fiscal Year:
  2003
• NORA Priority Area:
  Disease and Injury: Musculoskeletal Disorders of the Upper Extremities
• Performing Organization:
  West Virginia University, Morgantown, West Virginia
• Peer Reviewed:
  True
• Start Date:
  19920101
• Source Full Name:
  Muscle & Nerve
• End Date:
  20050531
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:986ec4a1a13418073a45f2397f32a21d14cd627ec90e5c986c51e5188f4bf9ca3e284b7315c6c6cd3d5f23b92b3cc00c4f78ffc3f9d985d8b6f7b2ca8a9d05e8
• Download URL:
  https://stacks.cdc.gov/view/cdc/190300/cdc_190300_DS1.pdf
• File Type:
  [PDF - 284.96 KB ]