Detection of 1-Hydroxypyrene as a Urine Biomarker of Human PAH Exposure Determined by Fluorescence and Solid-Matrix Luminescence Spectroscopy

Details

• Personal Author:
  Hurtubise RJ ; Ramasamy SM ; Weston A
• Description:
  A new solid-matrix luminescence method was developed for human biomonitoring that employs room-temperature fluorescence and phosphorescence for the detection and identification of 1-hydroxypyrene (1-OH-PY). Several high-performance liquid chromatography (HPLC) fractions from human urine samples were characterized by both solution fluorescence and solid-matrix luminescence. The solution fluorescence data and the solid-matrix luminescence data complemented each other. For example, in one of the fractions, the solution fluorescence showed the presence of tetrahydrotetrols of benzo[ a ]pyrene. However, in this fraction, 1-OH-PY was readily detected by solid-matrix luminescence. In addition, solution fluorescence showed that 1-OH-PY was not in some of the fractions, but solid-matrix luminescence showed that 1-OH-PY was present. Solid-matrix phosphorescence was very effective in detecting 1-OH-PY in the fractions where solution fluorescence and solidmatrix fluorescence indicated that 1-OH-PY was not present. The limits of detection were in the pmol/mL range by solution fluorescence and in the subpicomole range with solid-matrix luminescence. [Description provided by NIOSH]
• Subjects:
  Air Pollutants, Occupational Air Pollution Air Pollution, Indoor Chemistry Chemistry, Analytic Chromatography Environmental Exposure Environmental Health Environmental Monitoring Inhalation Exposure Occupational Health Particulate Matter Safety Urine

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• Keywords:
  Air-quality Air-quality-measurement Air-quality-monitoring Airborne-particles Analytical-chemistry Chromatographic-analysis Environmental-exposure Environmental-factors Environmental-health Exposure-levels Indoor-air-pollution Indoor-environmental-quality Inhalants Luminescence Urinalysis Urine-chemistry

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• ISSN:
  0003-7028
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  New York ; OSHA Region 2 ; OSHA Region 3 ; OSHA Region 5 ; OSHA Region 8 ; West Virginia ; Wisconsin ; Wyoming
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  51
• Issue:
  9
• NIOSHTIC Number:
  nn:20028055
• Citation:
  Appl Spectrosc 1997 Sep; 51(9):1377-1383
• Contact Point Address:
  Robert J. Hurtubise, Department of Chemistry, University of Wyoming, Laramie, Wyoming 82071-3838
• Federal Fiscal Year:
  1997
• Peer Reviewed:
  True
• Source Full Name:
  Applied Spectroscopy
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4232a0a18630b5a14cb19375ef06805b21c79e0b6b3dacac17febb40c6c0f4168b31683e528fbcbf80e35b07374e0857b1dc7017d25753331bc9de4301370833
• Download URL:
  https://stacks.cdc.gov/view/cdc/189754/cdc_189754_DS1.pdf
• File Type:
  [PDF - 201.54 KB ]