In Vitro Studies on the Genotoxicity of 2,4-Dichloro-6-Nitrophenol Ammonium (DCNPA) and Its Major Metabolite

Details

• Personal Author:
  Heng ZC ; Nath J ; Ong T
• Description:
  2,4-dichloro-6-nitrophenol ammonium (DCNPA) is used as a herbicide. However, information on the potential health hazards of DCNPA is limited. In a previous study, we found that DCNPA is genotoxic to Bacillus subtilis and yeast. Further studies were performed to determine whether DCNPA and its major metabolite, 2,4-dichloro-6-aminophenol (DCAP), can induce reverse mutations in Salmonella, gene mutations at the HPRT locus, sister chromatid exchanges (SCEs) and micronuclei (MN) in V79 cells. Results show that DCNPA does not produce a positive response for any endpoint at concentrations tested. However, treatment of V79 cultures with DCAP caused a significant increase in SCEs and MN in a concentration-dependent manner. These results indicate that DCAP damages DNA and causes chromosomal aberrations in V79 cells. Therefore, DCNPA could pose potential health hazards to populations exposed to this herbicide. [Description provided by NIOSH]
• Subjects:
  Chemistry Genetics Hazardous Substances Mutagens Occupational Health Risk Assessment Risk Factors Safety
• Keywords:
  Ames Test Author Keywords: 2,4-Dichloro-6-nitrophenol Ammonium Cell-alteration Cell-biology Cell-damage Cell-transformation Cellular-reactions Chemical-properties Chemical-reactions Gene-mutation Gene Mutation Genetic-factors Genotoxic-effects Health-hazards Herbicide Micronucleus Assay Mutation Risk-factors Sister Chromatid Exchange

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• ISSN:
  0027-5107
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DRDS - Division of Respiratory Disease Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  149-155
• Volume:
  368
• Issue:
  2
• NIOSHTIC Number:
  nn:20034334
• Citation:
  Mutat Res 1996 Jun; 368(2):149-155
• Contact Point Address:
  Genetics and Developmental Biology Program, College of Agriculture and Forestry, West Virginia University, Morgantown 26506-6108
• Federal Fiscal Year:
  1996
• Peer Reviewed:
  True
• Source Full Name:
  Mutation Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:13635b5f7540f9fa761e9fad9be160fbc50f01cd6c0056d4a65ae4fc808422d43ebbd78c243558511829d8977e71acaf7268d593e9d5b647f437d4373a687bee
• Download URL:
  https://stacks.cdc.gov/view/cdc/189581/cdc_189581_DS1.pdf
• File Type:
  [PDF - 542.41 KB ]