Eukaryotic Translation Elongation Factor 1delta Overexpressing Cells Are Resistant to Cadmium-Induced Toxicity and Apoptosis

Details

• Personal Author:
  Joseph P ; Othumpangat S ; Umbright C
• Description:
  The role of Eukaryotic translation elongation factor 1sigma (eEF1sigma) in cadmium-induced cytotoxicity and apoptosis was investigated. Transgenic cell line overexpressing the eEF1sigma gene were generated by transfecting HeLa cells with the expression vector pcDNA 3.1 containing human eEF1sigma cDNA. Stable transfectant cell line was developed using blasticidin (5mg/mL medium). Expression of eEF1sigma in the transgenic cell line was determined by real-time PCR and Western blot analysis. Cell line exhibiting 2 fold (eEF1sigma4) and 3 fold (eEF1sigma11) increases in eEF1sigma expression were developed. The vector alone transfected cell line served as the control. The transgenic cell line exhibited enhanced cell proliferation, overexpression of eEF1sigma, eIF4E and eEF2 genes compared with the control cell line. Cadmium-induced cytotoxicity was significantly lower in the eEF1sigma overexpressing cell line compared with the control. Cadmium induced apoptosis was also studied using the control and eEF1sigma overexpressing cell lines. The eEF1sigma overexpressing cell line exhibited significantly higher resistance to cadmium-induced apoptosis compared with the control cell line. Real-time PCR analysis of eEF1sigma overexpressing cell line exposed to cadmium showed significant induction of anti-apoptotic genes like Bad and Bcl-2, with a concurrent decrease in the expression of the pro-apoptotic gene, p53 compared with the control cell line. In summary, the eEF1sigma gene overexpressing cell line exhibited significant resistance to cadmium-induced cytotoxicity and apoptosis. [Description provided by NIOSH]
• Subjects:
  Cytotoxins Genetics Hazardous Substances Occupational Health Risk Assessment Risk Factors Safety
• Keywords:
  Cell-alteration Cell-function Cell-metabolism Cell-morphology Cell-transformation Cellular-structures Cytotoxic-effects Cytotoxicity Gene-mutation Genes Genetic-factors Genotoxic-effects Genotoxicity Mathematical-models Risk-analysis Risk-factors Statistical-analysis Toxic-effects

  More +
• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  396
• Volume:
  96
• Issue:
  1
• NIOSHTIC Number:
  nn:20031935
• Citation:
  Toxicologist 2007 Mar; 96(1):396
• CAS Registry Number:
  Cadmium (CAS RN 7440-43-9)
• Federal Fiscal Year:
  2007
• Peer Reviewed:
  False
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:7e5a458c11668679940aa6a5e5232c9a0a142edeb2803cc13d43822f9698a700899a9b4ab13ec59f3368f74154015234f296437e58d1d2fdc4b595c58a50153c
• Download URL:
  https://stacks.cdc.gov/view/cdc/186045/cdc_186045_DS1.pdf
• File Type:
  [PDF - 521.42 KB ]