Discrimination of Penicillium Isolates by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Fingerprinting

Details

• Personal Author:
  Beezhold, Donald H. ; Blanchere, Francoise M. ; Buskirk AD ; Green, Brett J. ; Hettick JM ; Janotka E ; Kashon ML ; Schmechel D ; Slaven, James E.
• Description:
  Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral 'fingerprints' for twelve Penicillium species. Prior to MALDI-TOF MS analysis, eight replicate cultures of each Penicillium species were subjected to three one-minute bead-beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contained abundant peaks in the range of m/z 5,000-20,000, and allowed unambiguous discrimination between species. In addition, a biomarker common to all Penicillium mass spectra was observed at m/z 13,900. Discriminant analysis using the MALDI-TOF MS data yielded classification error rates of 0% (i.e. 100% correct identification), indicating that MALDI-TOF MS data may be a useful diagnostic tool for the objective identification of Penicillium species of environmental and clinical importance. [Description provided by NIOSH]
• Subjects:
  Biomarkers Clinical Laboratory Techniques Diagnosis Environmental Health Fungi Mass Spectrometry Microbiology Occupational Health Safety Spectrum Analysis
• Keywords:
  Clinical-chemistry Clinical-tests Diagnostic-tests Environmental-factors Environmental-medicine Mass-spectrometry Microbial-test-systems Microorganisms Microscopic-analysis Spectrographic-analysis Spectroscopes Statistical-analysis

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• ISSN:
  0951-4198
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  22
• Issue:
  16
• NIOSHTIC Number:
  nn:20034322
• Citation:
  Rapid Commun Mass Spectrom 2008 Aug; 22(16):2555-2560
• Contact Point Address:
  Justin M. Hettick, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, ACIB, MS L-2040, 1095 Willowdale Road, Morgantown, WV 26505-2888
• Email:
  jhettick@cdc.gov
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Services
• Peer Reviewed:
  True
• Source Full Name:
  Rapid Communications in Mass Spectrometry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:73b2627ebf9da9479c4549ecb615b869c28fa506f2e8eb40ce598143e20e0d63487901f3e78185c44354d98b69b4a7c99b75ac6832e5fd1ae255c6e12d849717
• Download URL:
  https://stacks.cdc.gov/view/cdc/189570/cdc_189570_DS1.pdf
• File Type:
  [PDF - 121.79 KB ]