Discrimination of Aspergillus Isolates at the Species and Strain Level by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Fingerprinting

Details

• Personal Author:
  Beezhold, Donald H. ; Blanchere, Francoise M. ; Buskirk AD ; Green, Brett J. ; Hettick JM ; Janotka E ; Kashon ML ; Schmechel D ; Slaven, James E.
• Description:
  Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was used to generate highly reproducible mass spectral fingerprints for 12 species of fungi of the genus Aspergillus and 5 different strains of Aspergillus flavus. Prior to MALDI-TOF MS analysis, the fungi were subjected to three 1-min bead beating cycles in an acetonitrile/trifluoroacetic acid solvent. The mass spectra contain abundant peaks in the range of 5 to 20kDa and may be used to discriminate between species unambiguously. A discriminant analysis using all peaks from the MALDI-TOF MS data yielded error rates for classification of 0 and 18.75% for resubstitution and cross-validation methods, respectively. If a subset of 28 significant peaks is chosen, resubstitution and cross-validation error rates are 0%. Discriminant analysis of the MALDI-TOF MS data for 5 strains of A. flavus using all peaks yielded error rates for classification of 0 and 5% for resubstitution and cross-validation methods, respectively. These data indicate that MALDI-TOF MS data may be used for unambiguous identification of members of the genus Aspergillus at both the species and strain levels. [Description provided by NIOSH]
• Subjects:
  Biomarkers Fungi Mass Spectrometry Microbiology Occupational Health Safety Spectrum Analysis
• Keywords:
  Analytical-processes Mass-spectrometry Microbial-test-systems Microchemistry Microorganisms Microscopic-analysis Spectrographic-analysis Spectroscopes Statistical-analysis
• ISSN:
  0003-2697
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  276-281
• Volume:
  380
• Issue:
  2
• NIOSHTIC Number:
  nn:20034320
• Citation:
  Anal Biochem 2008 Sep; 380(2):276-281
• Contact Point Address:
  Justin M. Hettick, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Health Effects Laboratory Division, 1095 Willowdale Road, Morgantown, WV 26505-2888
• Email:
  jhettick@cdc.gov
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Services
• Peer Reviewed:
  True
• Source Full Name:
  Analytical Biochemistry
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:fa699015721b75eab5dcaf1ddcf8947e770614be4188d9a39a85fb2e61183f17f26654a159d93e0f5c6cc06ca62353b8d633a9bf09f216bf07f7c47a2613106f
• Download URL:
  https://stacks.cdc.gov/view/cdc/189568/cdc_189568_DS1.pdf
• File Type:
  [PDF - 357.57 KB ]