Antimony-Induced Alterations in Thiol Homeostasis and Adenine Nucleotide Status in Cultured Cardiac Myocytes

Details

• Personal Author:
  Mathias PI ; Snawder, John E. ; Tirmenstein MA ; Toraason M ; Wey HE
• Description:
  Cultured cardiac myocytes were exposed for up to 4 h to 50 and 100 µM potassium antimonyl tartrate (PAT). After 4 h, 50 and 100 µM PAT killed 14 and 33% respectively of the cardiac myocytes. PAT-induced alterations in both protein and nonprotein thiol homeostasis. Transient increases in oxidized glutathione disulfide (GSSG) levels were detected after cells were treated with 100 µM PAT for 2 h. After 4 h, both concentrations of PAT significantly depleted reduced glutathione (GSH) levels. Protein thiols levels were also decreased after a 2-h exposure to 50 and 100 µM PAT. Cells treated with 50 µM and 100 µM PAT had a 15% and 40% reduction respectively in protein thiols after 4 h. PAT also significantly inhibited glutathione peroxidase and pyruvate dehydrogenase activity in cardiac myocytes. Pyruvate dehydrogenase activity levels were inhibited as early as 1 h after cells were treated with both concentrations of PAT. Cardiac myocyte ATP levels were also decreased by PAT, but only after a 4-h exposure to 50 µM and 100 µM PAT. Decreases in cellular ATP levels paralleled PAT toxicity put appeared to be secondary to other cellular changes initiated by PAT exposure. [Description provided by NIOSH]
• Subjects:
  Animals Antimony Cardiovascular System Laboratories Metals Metals, Heavy Occupational Health Safety Sulfhydryl Compounds
• Keywords:
  Animal-studies Antimony-compounds Cardiac-function Heavy-metals Laboratory-animals Metal-compounds Metallic-compounds Thiols
• ISSN:
  0300-483X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  203-211
• Volume:
  119
• Issue:
  3
• NIOSHTIC Number:
  nn:20033942
• Citation:
  Toxicology 1997 May; 119(3):203-211
• CAS Registry Number:
  Antimony (CAS RN 7440-36-0) ; Glutathione (CAS RN 70-18-8)
• Federal Fiscal Year:
  1997
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:54a888f627cb28b8da16134d26a78fd66f9d66bf6cc86e742f811f608b54acd57dfad141cb204b2f60fa19f7023b74f22cf4fdcd3eca0f75bf14cb430f901f76
• Download URL:
  https://stacks.cdc.gov/view/cdc/189340/cdc_189340_DS1.pdf
• File Type:
  [PDF - 892.40 KB ]