Antimony-Induced Alterations in Thiol Homeostasis and Adenine Nucleotide Status in Cultured Cardiac Myocytes

Details

• Personal Author:
  Mathias PI ; Snawder, John E. ; Tirmenstein MA ; Toraason M ; Wey HE
• Description:
  Cultured cardiac myocytes were exposed for up to 4 h to 50 and 100 µM potassium antimonyl tartrate (PAT). After 4 h, 50 and 100 µM PAT killed 14 and 33% respectively of the cardiac myocytes. PAT-induced alterations in both protein and nonprotein thiol homeostasis. Transient increases in oxidized glutathione disulfide (GSSG) levels were detected after cells were treated with 100 µM PAT for 2 h. After 4 h, both concentrations of PAT significantly depleted reduced glutathione (GSH) levels. Protein thiols levels were also decreased after a 2-h exposure to 50 and 100 µM PAT. Cells treated with 50 µM and 100 µM PAT had a 15% and 40% reduction respectively in protein thiols after 4 h. PAT also significantly inhibited glutathione peroxidase and pyruvate dehydrogenase activity in cardiac myocytes. Pyruvate dehydrogenase activity levels were inhibited as early as 1 h after cells were treated with both concentrations of PAT. Cardiac myocyte ATP levels were also decreased by PAT, but only after a 4-h exposure to 50 µM and 100 µM PAT. Decreases in cellular ATP levels paralleled PAT toxicity put appeared to be secondary to other cellular changes initiated by PAT exposure. Description provided by NIOSH
• Subjects:
  • Animals
  • Antimony
  • Cardiovascular System
  • Laboratories
  • Metals
  • Metals, Heavy
  • Occupational Health
  • Safety
  • Sulfhydryl Compounds
• Keywords:
  • Antimony-compounds
  • Metal-compounds
  • Metallic-compounds
  • Heavy-metals
  • Cardiac-function
  • Thiols
  • Laboratory-animals
  • Animal-studies
• ISSN:
  0300-483X
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  203-211
• Volume:
  119
• Issue:
  3
• NIOSHTIC Number:
  nn:20033942
• Citation:
  Toxicology 1997 May; 119(3):203-211
• CAS Registry Number:
  • Antimony (CAS RN 7440-36-0)
  • Glutathione (CAS RN 70-18-8)
• Federal Fiscal Year:
  1997
• Peer Reviewed:
  True
• Source Full Name:
  Toxicology
• Download URL:
  https://stacks.cdc.gov/view/cdc/189340/cdc_189340_DS1.pdf
• File Type:
  [PDF - 892.40 KB]
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:54a888f627cb28b8da16134d26a78fd66f9d66bf6cc86e742f811f608b54acd57dfad141cb204b2f60fa19f7023b74f22cf4fdcd3eca0f75bf14cb430f901f76