Biomarker and Metabolite Analysis by HPLC/MS

Details

• Personal Author:
  B'Hymer, Clayton ; Cheever KL
• Description:
  High-performance liquid chromatography-mass spectrometry (HPLC-MS) is a powerful analytical technique widely used in recent years for the analysis of biomarkers and metabolites. Biomarker determination and quantification, whether metabolic or adducted biomolecules, are commonly used to evaluate exposure and support biomonitoring research, especially in the area of occupational exposure and health. Some of the common problems and strategies of HPLC-MS biomarker analysis involve matrix effects, the use of isotope labeled internal standard compounds and sample clean-up; usually all of these factors must be evaluated within the development phase of an analysis procedure. Specific examples of biomarker analysis using HPLC-MS include acrylamide, aromatic compounds, and 1-bromopropane, and these examples are discussed in detail. [Description provided by NIOSH]
• Subjects:
  Biomarkers Chemistry Chemistry, Analytic Occupational Health Safety
• Keywords:
  Analytical-chemistry Analytical-methods Analytical-processes Sample-preparation Sampling-methods
• ISBN:
  9780824727888
• Publisher:
  Taylor and Francis
• Document Type:
  Text
• Genre:
  Book
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  1-10
• NIOSHTIC Number:
  nn:20033732
• Citation:
  Encyclopedia of chromatography, 3rd edition. Cazes J, ed. Boca Raton, FL: Taylor and Francis, Online-supplement, 2008 Jan; :1-10
• CAS Registry Number:
  1-Bromopropane (CAS RN 106-94-5) ; Acrylamide (CAS RN 79-06-1)
• Editor(s):
  Cazes J
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Healthcare and Social Assistance ; Services
• Peer Reviewed:
  False
• Source Full Name:
  Encyclopedia of chromatography, 3rd edition
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:4bfdd42e025254a38fe38f7c6be542678fb050b664549890f7d335a8f41ba51cd15804d4161dc4b834af5834c2eafd8a3139e43a58eb32da8e939b2d7d3ff75b
• Download URL:
  https://stacks.cdc.gov/view/cdc/189193/cdc_189193_DS1.pdf
• File Type:
  [PDF - 4.63 MB ]