U.S. flag An official website of the United States government.
Official websites use .gov

A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS

A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

i

Evaluation of Extraction Conditions and Use of HPLC-MS for the Simultaneous Determination of Acrylamide and Its Primary Metabolite, N-Acetyl-S-(2-Carbamoylethyl)cysteine, in Human Urine

Public Domain


Details

  • Personal Author:
  • Description:
    Extraction conditions were evaluated for the simultaneous determination of acrylamide and its primary metabolite, N-Acetyl-S-(2-carbamoylethyl)cysteine (NACEC), in human urine. Acrylamide is an animal carcinogen and a human neurotoxicant; and it is widely used within industry. The toxicity of acrylamide makes it a health concern, and the use of its metabolite, NACEC, as a biomarker of exposure would be of value in the prevention of occupational diseases. Sample preparation studies evaluating several different types of solid-phase extraction (SPE) cartridges and different buffered or acidic matrices of standing urine samples were conducted. Measurement of acrylamide and NACEC was by reversed-phase high performance liquid chromatography (HPLC) with a mobile phase gradient. Detection for quantification was by single ion monitoring using electrospray mass spectrometry (MS). A basic method validation, using the final optimized SPE conditions, was conducted. Recovery studies of fortified urine samples at various concentration levels demonstrated good accuracy and precision; recovery varied between 97 and 108% for acrylamide and with relative standard deviations (RSD) of 7.6% or less. Recovery for the NACEC metabolite varied between 97 and 102% with RSD of 10% or less. The limit of detection (LOD) for the optimized procedure was found to range from 0.02 to 0.03 mg/mL for acrylamide and 0.1 to 0.2 mg/mL for NACEC in urine, using two chromatographic columns of different production lots. [Description provided by NIOSH]
  • Subjects:
  • Keywords:
  • ISSN:
    1082-6076
  • Document Type:
    Text
  • Genre:
    Journal Article
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Division:
    DART - Division of Applied Research and Technology
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Volume:
    30
  • Issue:
    9
  • NIOSHTIC Number:
    nn:20031830
  • Citation:
    J Liq Chromatogr Relat Technol 2007 May; 30(9-10):1303-1316
  • Contact Point Address:
    C. B'Hymer, U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Taft Laboratory, 4676 Columbia Parkway, Cincinnati, Ohio 45226, USA
  • Email:
    cbhymer@cdc.gov
  • CAS Registry Number:
    Acrylamide (CAS RN 79-06-1)
  • Federal Fiscal Year:
    2007
  • NORA Priority Area:
  • Peer Reviewed:
    True
  • Source Full Name:
    Journal of Liquid Chromatography & Related Technologies
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:475b96aa5618fe1d37d108cdd991a030893bc8b27e995b56c53824849a97568474adb39ef87101fff9eabbef59600fed290c10f8ac82b35eb81cb7ce22da5332
  • Download URL:
  • File Type:
    Filetype[PDF - 99.94 KB ]
PREVIOUS
ON THIS PAGE
Details
CDC STACKS serves as an archival repository of CDC-published products including scientific findings, journal articles, guidelines, recommendations, or other public health information authored or co-authored by CDC or funded partners.

As a repository, CDC STACKS retains documents in their original published format to ensure public access to scientific information.
BACK TO TOP