Creatinine Measurements in 24 H Urine by Liquid Chromatography-Tandem Mass Spectrometry

Details

• Personal Author:
  Gee SJ ; Hammock BD ; Park E-K ; Schenker MB ; Watanabe T
• Description:
  A simple, sensitive, and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for determining urinary creatinine was developed and used to evaluate 24 h urine samples collected during an exposure study. Urine (1 microL) was diluted with methanol and then directly applied to LC-MS/MS. Under electrospray ionization (ESI) conditions, the transition molecules of creatinine and creatinine- d3 were observed at m/z 114 > 44 and m/z 117 > 47, respectively. The retention time of creatinine was 0.59 min. The linear range was 1-2000 ng/mL, with a detection limit in urine of 1 ng/mL. LC-MS/MS and colorimetric end-point methods were significantly associated (R2 = 0.8785, p < 0.0001). The LC-MS/MS method to determine creatinine in 24 h urine samples had shorter retention times, was more sensitive, reliable, reproducible, simple, selective, and used a smaller sample size than other LC-MS/MS or commercial methods. [Description provided by NIOSH]
• Subjects:
  Agriculture Occupational Health Safety Urine
• Keywords:
  Agricultural Chemicals Liquid Chromatography Mass Spectrometry Sampling Methods Urinalysis Urine Chemistry
• ISSN:
  0021-8561
• Document Type:
  Text
• Funding:
  Cooperative Agreement
• Genre:
  Journal Article
• Place as Subject:
  California ; OSHA Region 9
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  333-336
• Volume:
  56
• Issue:
  2
• NIOSHTIC Number:
  nn:20033680
• Citation:
  J Agric Food Chem 2008 Jan; 56(2):333-336
• Contact Point Address:
  Department of Entomology and UCD Cancer Research Center, University of California, Davis, California 95616
• CAS Registry Number:
  Methanol (CAS RN 67-56-1)
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Agriculture, Forestry and Fishing
• Performing Organization:
  University of California - Davis
• Peer Reviewed:
  True
• Start Date:
  20010930
• Source Full Name:
  Journal of Agricultural and Food Chemistry
• End Date:
  20270929
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:58c672f63f119ff999a272f44eea01ec97524d12df110085fe10de47b3c31f236864a8bd9232d60a1aaae797a288ba18450551c5a060950a5175781299ce82cf
• Download URL:
  https://stacks.cdc.gov/view/cdc/189159/cdc_189159_DS1.pdf
• File Type:
  [PDF - 113.75 KB ]