Identification of a major human urinary metabolite of alachlor by LC- MS/MS

Details

• Personal Author:
  Driskell WJ ; Hill RH Jr. ; Hines CJ ; Hull, R. DeLon ; Shealy DB
• Description:
  Urinary metabolites of the herbicide alachlor (15972608) were identified in occupationally exposed subjects by liquid chromatography coupled to mass spectrometry. Urine samples were collected from four workers; daughter and parent scanning modes were then utilized to identify alachlor metabolites. On the basis of mass spectrometry and chromatographic behavior, the major metabolite was identified as alachlor-mercapturate. A method was then developed to measured this metabolite in urine. In the four urinary samples, alachlor-mercapturate levels ranged from 25 to 62% of the concentration of the alachlor hydrolysis product, 2,6-diethylaniline (579668) (DEA). No other alachlor metabolites were positively identified. The authors conclude that identification of alachlor- mercapturate allows for the development of novel methods to assess alachlor exposure and enable more effective biomonitoring; a method based on measurement of this metabolite should prove to be simpler, safer, and more specific than the previously used DEA method. [Description provided by NIOSH]
• Subjects:
  Chromatography Herbicides Mass Spectrometry Occupational Exposure Occupational Health Safety Urine
• Keywords:
  Analytical-methods Biological-monitoring Chromatographic-analysis Mass-spectrometry NIOSH-Author Occupational-exposure Urinalysis Urine-chemistry
• ISSN:
  0007-4861
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Georgia ; Ohio ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DBBS - Division of Biomedical and Behavioral Sciences ; DSHEFS - Division of Surveillance, Hazard Evaluations, and Field Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  56
• Issue:
  6
• NIOSHTIC Number:
  nn:00231592
• Citation:
  Bull Environ Contam Toxicol 1996 Jun; 56(6):853-859
• Contact Point Address:
  W. J. Driskell, National Center for Environmental Health, Centers for Disease Control and Prevention (CDC), U.S. Department of Health and Human Services, Atlanta, Georgia 30333, USA
• CAS Registry Number:
  2,6-Diethylaniline (CAS RN 579-66-8) ; Alachlor (CAS RN 15972-60-8)
• Federal Fiscal Year:
  1996
• Peer Reviewed:
  True
• Source Full Name:
  Bulletin of Environmental Contamination and Toxicology
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:d1a0d3583484ecec9c9248e31f29cba744de58c1aa73387c9add9b106e03bf068404a790fdc201dce8d04a3e40d587c84d98d96c703df7b8487d71931944e7e2
• Download URL:
  https://stacks.cdc.gov/view/cdc/204870/cdc_204870_DS1.pdf
• File Type:
  [PDF - 56.60 KB ]