Use of Labeled Single Walled Carbon Nanotubes to Study Acute Translocation from the Lungs

Details

• Personal Author:
  Battelli LA ; Castranova, Vincent ; Mercer RR ; Scabilloni JF ; Wang L ; Battelli LA ; Castranova, Vincent ; Mercer RR ; Scabilloni JF ; Wang L
• Description:
  Single walled carbon nanotubes (SWCNT) have a small size and unique physicalchemical properties which suggest that novel particles may translocate to systemic organs after deposition in the lungs. In this study, gold particles (10nm) were used to label a highly dispersed preparation of SWCNT with a mean geometric diameter of 1.1 microns. The gold labeled SWCNT were delivered to the lungs by pharyngeal aspiration to C57BL/6 mice. Neutron activation analysis (NAA) of lung, blood and other organs was carried out at various times after aspiration to determine if the gold labeled SWCNT translocated out of the lungs. Six mice per group were studied at 1 hour, 1 day and 3 days after exposure to a single dose of 50 ug of gold labeled SWCNT. A separate group was given gold particles without SWCNT. A phosphate buffered saline (PBS) aspiration group served as the negative control. At sacrifice the lungs, heart, brain, liver, kidneys and a blood sample were taken for analysis of gold content by NAA. Gold content of the PBS aspiration group was negligible. In the gold labeled SWCNT group lung gold content at 1 day was 65 percent of that at 1 hour and not significantly different from the content at 3 days. The heart, brain, liver, kidney and blood samples from the gold labeled SWCNT groups contained less than 1 part in 200 of the total dose and were not statistically different from the PBS aspiration group at 1 hour, 1 day and 3 days. The results indicate that SWCNT do not readily translocate from the lungs. [Description provided by NIOSH]
• Subjects:
  Dust Irritants Laboratories Lung Lung Diseases Lymphatic System Occupational Health Particulate Matter Respiratory Hypersensitivity Respiratory System Respiratory Tract Diseases Safety

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• Keywords:
  Laboratory-animals Lung-burden Lung-function Lung-irritants Lymphatic-system-disorders Nanotechnology Particle-aerodynamics Particulate-dust Particulate-sampling-methods Pulmonary-disorders Pulmonary-system-disorders Respirable-dust Respiratory-hypersensitivity Respiratory-irritants

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Abstract or Summary
• Place as Subject:
  OSHA Region 10 ; OSHA Region 3 ; Washington ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH) ; National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division ; HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health ; Workplace Safety & Health
• Location:
  North America ; North America
• Volume:
  102
• Issue:
  1
• NIOSHTIC Number:
  nn:20033609
• Citation:
  Toxicologist 2008 Mar; 102(1):286
• CAS Registry Number:
  Carbon (CAS RN 7440-44-0) ; Gold (CAS RN 7440-57-5) ; Carbon (CAS RN 7440-44-0) ; Gold (CAS RN 7440-57-5)
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Manufacturing ; Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 47th Annual Meeting and ToxExpo, March 16-20, 2008, Seattle, Washington
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:8c06413c9c3a3149c576debb2fad217c92f810f63406f21ab0693d95ff17497ebfc83c4ee293d2854eb1ba29edda5f5a98fbead5bff941da6474059187ffc081
• Download URL:
  https://stacks.cdc.gov/view/cdc/189106/cdc_189106_DS1.pdf
• File Type:
  [PDF - 7.65 MB ]