Evidence for Cross Linking of Neurofilament (NF) Proteins in Rat Spinal Cord Following Inhalation Exposure to Carbon Disulfide

Details

• Personal Author:
  Abou-Donia MB ; Viana ME ; Wilmarth KR
• Description:
  Chronic exposure to CS2 produces morphological changes in the distal regions of long myelinated fibers characterized by perinodal accumulation of neurofilaments in axonal swellings. CS2 has also been reported to bind neuronal proteins in vivo and covalently crosslink bovine serum albumin in vitro. In this study, male Sprague-Dawley rats were exposed to 50, 100, or 200 ppm CS2 via inhalation 12h/day for 140 consecutive days. 24h following the end of treatment, animals were sacrificed and cytoskeletal proteins were isolated from spinal cords of individual animals. Proteins were resolved by SDSPAGE and stained with Coomassie Blue, or transferred to nitrocellulose and probed with monoclonal antisera against NF200, 160 and 68kD. We observed a significant decrease in the intensity of protein staining and immunoreactivity of all three NF protein bands as well as decreased immunoreactive breakdown products in animals exposed to 200 ppm CS2. A dose dependent increase in the appearance of multiple bands migrating between 260 and 400 kD with immunoreactivity against NF 160 and NF68 antibodies was also observed. The relative increase in immunoreactivity against the proteins with increasing levels of exposure was NFI6Q>NF68. These results suggest that CS2 induced cross linking of NF proteins may be involved in the aggregation of NFcharacteristic of this neuropathy. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Monitoring Hazardous Substances Laboratories Nervous System Occupational Exposure Occupational Health Safety Toxicology
• Keywords:
  Animal-studies Central-nervous-system Chronic-exposure Chronic-toxicity Exposure-assessment Exposure-levels Laboratory-animals Laboratory-testing Toxic-effects Toxins
• ISSN:
  0731-9193
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  1
• NIOSHTIC Number:
  nn:20035549
• Citation:
  Toxicologist 1994 Mar; 14(1):205
• CAS Registry Number:
  Carbon disulfide (CAS RN 75-15-0)
• Federal Fiscal Year:
  1994
• Performing Organization:
  Duke University Medical Center, Durham, North Carolina
• Peer Reviewed:
  False
• Start Date:
  19790401
• Source Full Name:
  The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6cfd929261166f827c1c111141982374ef4173dca9698685e72e09068c18020422d00bce65f6e5c59f42b4adb97df5fe135821a937d25afcf34958c5ad046058
• Download URL:
  https://stacks.cdc.gov/view/cdc/187373/cdc_187373_DS1.pdf
• File Type:
  [PDF - 236.74 KB ]