Hair as a Target Tissue for Protein-Bound Pyrroles Following Subchronic Intra Peritoneal Injections of 2,5-Hexanedione

Details

• Personal Author:
  Abdel-Rahman SM ; Abou-Donia MB ; Johnson JD ; Lack L
• Description:
  Studies were carried out to ascertain the feasible use of body hair as a biological marker for chronic exposure to industrial neurotoxicants that yield the metabolite, 2,5-hexanedione (2,5-HD), i.e., n-hexane and methyl-n-butyl ketone. 2,5-HD is capable of forming N-substituted pyrroles by reacting with primary amines which include amino acids as well as the E-amino groups of lysine-containing peptides. Male SpragueDawley rats were given daily ip. injections of 50 mg/kg 2,5-HD for 45 days. At intervals, hair samples distant from the site of injection of treated animals were taken and showed staining which was positive for the presence of pyrroles with Ehrlich's reagent (p-N,N-dimethylaminobenzaldehyde), whereas control animals were negative. In addition, proteins were solubilized from these samples. The protein solutions from the treated samples tested positive for the presence of pyrroles with Ehrlich's reagent and upon spectral analysis yielded an absorbance peak of 530 nm which is in the range characteristic of this type of pyrrole. The extent of positive staining for pyrroles in the vibrissae progressed linearly with time. These findings suggest the potential use of hair as an indicator for chronic exposure to this class of potential industrial neurotoxic chemicals and as a complement to the urinary analysis which is used to confirm recent exposure. [Description provided by NIOSH]
• Subjects:
  Animals Environmental Monitoring Hazardous Substances Laboratories Nervous System Occupational Exposure Occupational Health Safety Toxicology
• Keywords:
  Animal-studies Central-nervous-system Chronic-exposure Chronic-toxicity Exposure-assessment Exposure-levels Laboratory-animals Laboratory-testing Neurotoxic-effects Neurotoxins Pyrroles Toxic-effects Toxins

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• ISSN:
  0731-9193
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Abstract or Summary
• Place as Subject:
  North Carolina ; OSHA Region 4
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  1
• NIOSHTIC Number:
  nn:20035553
• Citation:
  Toxicologist 1994 Mar; 14(1):360
• CAS Registry Number:
  2-Hexanone (CAS RN 591-78-6) ; Hexane (CAS RN 110-54-3)
• Federal Fiscal Year:
  1994
• Performing Organization:
  Duke University Medical Center, Durham, North Carolina
• Peer Reviewed:
  False
• Start Date:
  19790401
• Source Full Name:
  The Toxicologist. Society of Toxicology 33rd Annual Meeting, March 13-17, 1994, Dallas, Texas
• End Date:
  19980331
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:bdb1bae411a6179733aec195caebe83bc79fb6b9c823c8c2a1b9029c1a682fdbb1f15e3f187600e61b7692a71533aca458c6b7ee6569433b9d0d3b49fd1773f8
• Download URL:
  https://stacks.cdc.gov/view/cdc/187377/cdc_187377_DS1.pdf
• File Type:
  [PDF - 233.41 KB ]