Comparison of Carbon Nanotube-Induced Cytotoxicity in A549 and Normal Human Bronchial Epithelial (NHBE) Cells

Details

• Personal Author:
  Hulderman T ; Simeonova PP ; Walker V
• Description:
  The most attractive features of nanomaterials including their small size, large surface area, and reactivity might also be the main factors for their toxicity. Pulmonary bronchial epithelial cells are a potential target for toxicity during respiratory exposure. The tumor cell line A549, a pulmonary human type-II like epithelial cells, is widely used for evaluation of particle toxicity. We hypothesized that A549 cells are more resistant to toxicity compared to the normal epithelial cells. To test this hypothesis we examined the dose-dependent cytotoxicity effects of single-walled (SWCNT) and multi-walled (MWCNT) carbon nanotubes on NHBE and A549 cells using lactate dehydrogenase (LDH) release and the cell proliferation/viability WST-1 assays. We found that NHBE exhibited marked cytotoxicity, similar to that observed with hydrogen peroxide treatment (positive control), consistently by 24 hours post-exposure to high concentrations [50-150mu g/106 cells (1.5-4.5mu g/ml)] of CNT. In comparison, exposure of A549 cells to similar concentration of CNT resulted in only a milder response. Overall, the results presented here indicate that SWCNT or MWCNT induce cytotoxicity in human lung epithelial cells in a time and dose-dependent manner. Furthermore, we demonstrate a large variation between the particle-induced cytotoxic responses in A549 cells and normal primary epithelial cells. [Description provided by NIOSH]
• Subjects:
  Biological Factors Cytology Environmental Monitoring Hazardous Substances Irritants Lung Lung Diseases Microbiology Occupational Health Particulate Matter Peroxides Respiratory System Respiratory Tract Diseases Safety Toxicology

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• Keywords:
  Biological-effects Biological-factors Cell-biology Cell-damage Cell-morphology Cellular-function Cellular-reactions Exposure-assessment Exposure-levels Hydroperoxides Inhalation-studies Lung-cells Lung-irritants Microscopic-analysis Microscopy Nanotechnology Particle-aerodynamics Particulates Pulmonary-disorders Pulmonary-system-disorders Respiratory-irritants Respiratory-system-disorders Statistical-analysis Toxic-effects Toxicopathology

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  108
• Issue:
  1
• NIOSHTIC Number:
  nn:20035313
• Citation:
  Toxicologist 2009 Mar; 108(1):461
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Manufacturing
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 48th Annual Meeting and ToxExpo, March 15-19, 2009, Baltimore, Maryland
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:dd7322a6d765be68d51edbbbbdade9ca6136470eb2ec46e5113d12808dca967b5be48cb9b9784cd6f575871b5011e55fbdd46e342909dced36de4a2f03302b9d
• Download URL:
  https://stacks.cdc.gov/view/cdc/187221/cdc_187221_DS1.pdf
• File Type:
  [PDF - 1.87 MB ]