Development of an HPLC – MS Procedure for the Quantification of N-Acetyl-S-(N-Propyl)-L-Cysteine, the Major Urinary Metabolite of 1-Bromopropane in Human Urine

Details

• Personal Author:
  B'Hymer, Clayton ; Cheever KL ; Hanley, Kevin W. ; Lynch DW ; Marlow, Kate L.
• Description:
  An analytical procedure was developed for the detection and quantification of N-acetyl-S-(npropyl)- l-cysteine (n-propylmercapturic acid, AcPrCys), a metabolite and biomarker for exposure to 1-bromopropane (1-BP). 1-BP is used as an industrial solvent and exposure is a health concern for industrial workers due to its toxicity. It has been associated with neurological disorders in both animals and humans. Urine sample preparation for the determination of AcPrCys consisted of solid phase extraction (SPE). Urine samples on preconditioned SPE (C18) columns were washed with 40% methanol/60% water solution prior to elution with acetone. Quantificationwas by means of a liquid chromatograph (LC) equipped with a mass spectrometer (MS) using an Aqua 3mu m C18 300A column and [d7]-AcPrCys was used as internal standard. Electrospray ionization (ESI)was used with the MS operated in the negative ion mode and selected ion monitoring (SIM) at m/z 204 for AcPrCys and m/z 211 for [d7]-AcPrCys. Demonstrated recovery of urine samples fortified at multiple levels (0.625-10mu g/ml) varied between 96 and 103% of theory with relative standard deviations (RSD) of 6.4% or less. The limit of detection (LOD) for the procedure was approximately 0.01mu g/ml AcPrCys in urine. These data will be discussed as well as other factors of the development of this test procedure. [Description provided by NIOSH]
• Subjects:
  Animals Biochemistry Biological Factors Biomarkers Chemistry Chromatography Energy Metabolism Environmental Exposure Hazardous Substances Hypersensitivity Manufacturing And Industrial Facilities Nervous System Nervous System Diseases Occupational Health Safety Solvents Toxicology Urine Workplace

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• Keywords:
  Author Keywords: 1-Bromopropane Biological-effects Biological-factors Biological-monitoring Biological-transport Chemical-hypersensitivity Chemical-properties Chemical-reactions Exposure-levels Health-hazards HPLC-MS Humans Industrial-equipment Industrial-exposures Industrial-hazards Industrial-health-programs Industrial-processes Ion-exchange-chromatography Ionization Metabolic-study Metabolites Neurological-diseases Neurological-reactions Neurological-system Neurophysiology Neurotoxic-effects Quantitative-analysis Toxic-effects Toxicopathology Toxins Urinalysis Work-areas Work-environment Worker-health Workplace-studies

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• ISSN:
  1570-0232
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  Ohio ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology ; DSHEFS - Division of Surveillance, Hazard Evaluations, and Field Studies
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  877
• Issue:
  8
• NIOSHTIC Number:
  nn:20035157
• Citation:
  J Chromatogr B 2009 Mar; 877(8-9):827-832
• Contact Point Address:
  C. B'Hymer, U.S. Department of Health and Human Services, Centers for Disease Control and Prevention, National Institute for Occupational Safety and Health, Division of Applied Research and Technology, 4676 Columbia Parkway, Cincinnati, OH 45226
• Email:
  cbhymer@cdc.gov
• CAS Registry Number:
  1-Bromopropane (CAS RN 106-94-5)
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Services ; Healthcare and Social Assistance
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:9ed194910091081a8495d9522f32db431d38ad100f94becd140622cda00312e46629267361e216f9f3a794d7285f46e78d68260be3526956218f0bbdc6034657
• Download URL:
  https://stacks.cdc.gov/view/cdc/187099/cdc_187099_DS1.pdf
• File Type:
  [PDF - 232.96 KB ]