Mass-Spectrometric Analysis of Hydroperoxy- and Hydroxy-Derivatives of Cardiolipin and Phosphatidylserine in Cells and Tissues Induced by Pro-Apoptotic and Pro-Inflammatory Stimuli

Details

• Personal Author:
  Bayir H ; Greenberger JS ; Jung MY ; Kagan VE ; Kochanek PM ; Pitt B ; Shvedova AA ; Tungekar MA ; Tyurin VA ; Tyurina Y ; Wasserloos KJ
• Description:
  Oxidation of two anionic phospholipids - cardiolipin (CL) in mitochondria and phosphatidylserine (PS) in extramitochondrial compartments - is important signaling event, particularly during the execution of programmed cell death and clearance of apoptotic cells. Quantitative analysis of CL and PS oxidation products is central to understanding their molecular mechanisms of action. We combined the identification of diverse phospholipid molecular species by ESI-MS with quantitative assessments of lipid hydroperoxides using a fluorescence HPLC-based protocol. We characterized CL and PS oxidation products formed in a model system (cyt c/H2O2), in apoptotic cells (neurons, pulmonary artery endothelial cells) and mouse lung under inflammatory/oxidative stress conditions (hyperoxia, inhalation of single walled carbon nanotubes). Our results demonstrate the usefulness of this approach for quantitative assessments, identification of individual molecular species and structural characterization of anionic phospholipids that are involved in oxidative modification in cells and tissues. [Description provided by NIOSH]
• Subjects:
  Animals Biochemistry Biological Factors Energy Metabolism Nervous System Occupational Health Oxidants Safety Workplace
• Keywords:
  Biological-effects Biological-factors Biological-monitoring Cell-biology Metabolic-study Metabolites Molecular-biology Nanotechnology Neurological-reactions Oxidation Oxidative-metabolism Quantitative-analysis Work-areas Work-environment Worker-health Workplace-studies

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• ISSN:
  1570-0232
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; Pennsylvania ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  877
• Issue:
  26
• NIOSHTIC Number:
  nn:20035853
• Citation:
  J Chromatogr B 2009 Sep; 877(26):2863-2872
• Contact Point Address:
  Vladimir A. Tyurin, Center for Free Radical and Antioxidant Health, University of Pittsburgh, 100 Technol Dr, Suite 323, Pittsburgh, PA 15219
• Email:
  vtyurin@pitt.edu
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Manufacturing
• Performing Organization:
  University of Pittsburgh at Pittsburgh
• Peer Reviewed:
  True
• Start Date:
  20050701
• Source Full Name:
  Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
• End Date:
  20160630
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:6d514f7fde94296653b3f031a684a9d976a318e5bf2b5ad60dc4baef519276797a6b1473b5acb81d49972c66dd4f1054820b705c8062b2ffeed73496903c07a8
• Download URL:
  https://stacks.cdc.gov/view/cdc/187546/cdc_187546_DS1.pdf
• File Type:
  [PDF - 895.38 KB ]