Single Pre-Exposure to a High Dose of Zymosan Enhances Lung Defense Mechanisms and Accelerates the Pulmonary Clearance of a Bacterial Pathogen in Rats

Details

• Personal Author:
  Antonini JM ; Roberts, Jennifer R. ; Young SH
• Description:
  The present study examines the effects of pre-exposure to zymosan (a 1 --> 3-beta-glucan from baker yeast) on lung defense against bacterial infection. Rats received a single dose of zymosan A (0.6, 1.2, or 2.5 mg/kg body weight [bw]) or vehicle control (saline) via intratracheal instillation 3 days prior to intratracheal inoculation with 5 x 10(5) Listeria monocytogenes. Left lungs were homogenized and cultured to assess bacterial clearance, and bronchoalveolar lavage was performed on the right lungs to monitor lung inflammation and injury. Prior to bacterial infection, zymosan exposure resulted in elevated inflammation and oxidant production in the lungs. Zymosan treatment followed by infection led to an accelerated pulmonary clearance of bacteria when compared to the saline control group in a dose-dependent fashion. In addition, lower levels of injury and inflammation were associated with the enhanced bacteria clearance observed in zymosan-infected rats. Our findings suggest that zymosan exposure may enhance the lung immune response by activating alveolar macrophages prior to infection, and stimulating T cells involved in the adaptive immune response early after infection, thus resulting in a heightened pulmonary immune response. [Description provided by NIOSH]
• Subjects:
  Bacterial Infections Immune System Lung Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Alveolar-cells Author Keywords: 1-->3-beta-glucans Bacteria Bacterial-infections Bactericides Cell-biology Cell-function Cell-metabolism Cellular-reactions Cellular-uptake Immune-reaction Immune-system Immunology Listeria Monocytogenes Lung Clearance Lung-cells Lung-function Lung-irritants Pulmonary-clearance Pulmonary-function Pulmonary Inflammation Respiratory-infections Zymosan

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• ISSN:
  0190-2148
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  34
• Issue:
  9
• NIOSHTIC Number:
  nn:20034725
• Citation:
  Exp Lung Res 2008 Nov; 34(9):559-578
• Contact Point Address:
  Shih-Houng Young, Health Effects Laboratory Division, National Institute for Occupational Safety and Health, 1095 Willowdale Road, M-S 2015, Morgantown, WV 26505
• Email:
  sby5@cdc.gov
• Federal Fiscal Year:
  2009
• NORA Priority Area:
  Construction ; Manufacturing
• Peer Reviewed:
  True
• Source Full Name:
  Experimental Lung Research
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c74865147b0647a99c4d87c3897fb56a1234d59446953ae828b1f7cc7b350ebff97cb0c19d74fe30e12ac9daea2e46deb1a8fea210d483e55878688cfc3add63
• Download URL:
  https://stacks.cdc.gov/view/cdc/186827/cdc_186827_DS1.pdf
• File Type:
  [PDF - 1.04 MB ]