Vibration Causes Acute Vascular Injury in a Two-Step Process: Vasoconstriction and Vacuole Disruption

Details

• Personal Author:
  Bain JL ; Eddinger TJ ; Govindaraju SR ; Riley, Danny A.
• Description:
  Hand-arm vibration syndrome is a vasospastic and neurodegenerative occupational disease. In the current study, the mechanism of vibration-induced vascular smooth muscle cell (SMC) injury was examined in a rat-tail vibration model. Tails of male Sprague Dawley rats were vibrated continuously for 4 hr at 60 Hz, 49 m/s(2) with or without general anesthesia. Ventral tail arteries were aldehyde fixed and embedded in epoxy resin to enable morphological analysis. Vibration without anesthesia caused vasoconstriction and vacuoles in the SMC. Anesthetizing rats during vibration prevented vasoconstriction and vacuole formation. Exposing tail arteries in situ to 1 mM norepinephrine (NE) for 15 min induced the greatest vasoconstriction and vacuolation. NE induced vacuoles were twice as large as those formed during vibration. When vibrated 4 hr under anesthesia after pretreatment with NE for 15 min, the SMC lacked vacuoles and exhibited a longitudinal banding pattern of dark and light staining. The extracellular matrix was filled with particulates, which were confirmed by electron microscopy to be cellular debris. The present findings demonstrate that vibration-induced vasoconstriction (SMC contraction) requires functioning central nervous system reflexes, and the physical stress of vibration damages the contracted SMC by dislodging and fragmenting SMC vacuoles. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Musculoskeletal Diseases Musculoskeletal System Nervous System Nervous System Diseases Occupational Health Safety Vibration
• Keywords:
  Animal-studies Author Keywords: Hand-arm Vibration Biological-effects Biological-monitoring Laboratory-animals Musculoskeletal-system Musculoskeletal-system-disorders Neurovascular-disorders Norepinephrine Rat-tail Artery Smooth Muscle Cells Vibration-control Vibration-disease Vibration-effects Vibration-exposure

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• ISSN:
  1932-8486
• Document Type:
  Text
• Funding:
  Grant
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 5 ; Wisconsin
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  29
• Issue:
  8
• NIOSHTIC Number:
  nn:20034583
• Citation:
  Anat Rec 2008 Aug; 291(8):999-1006
• Contact Point Address:
  Danny A. Riley, Department of Cell Biology, Neurobiology & Anatomy, 8701 Watertown Plank Road, Medical College of Wisconsin, Milwaukee, WI 53226
• Email:
  dariley@mcw.edu
• Federal Fiscal Year:
  2008
• NORA Priority Area:
  Manufacturing ; Wholesale and Retail Trade
• Performing Organization:
  Medical College of Wisconsin, Milwaukee, Wisconsin
• Peer Reviewed:
  True
• Start Date:
  19990901
• Source Full Name:
  Anatomical Record
• End Date:
  20160831
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:cfddda94da48586fca20d9cd66f098c64d75ba0c5788de1f39ba94031708da4b50b26c932980e3436165a5159445bc8e6ce52548f264bb763fda9c0eda2ad590
• Download URL:
  https://stacks.cdc.gov/view/cdc/186740/cdc_186740_DS1.pdf
• File Type:
  [PDF - 856.41 KB ]