Lung Surfactant Decreases IL-1 and TNF-A Production at the Post-Transcriptional Level in LPS-Stimulated Alveolar Macrophages

Details

• Personal Author:
  Bowman L ; Meighan T ; Rao KMK
• Description:
  We have shown previously that lung surfactant inhibits nitric oxide production at the post-transcriptional level in lipopolysaccharide(LPS)-stimulated rat alveolar macrophages (Miles et al., Am.J.Physiol. 276:Ll86, 1999). In this study, we examined the effect of lung surfactant (200 microg phospholipid/ml), isolated from rat lung lavage, on the production of two pro-inflammatory cytokines, IL-1B and TNF-a, by LPS-stimulated rat alveolar macrophages. After 22 h incubation with LPS plus lung surfactant, IL-1B and TNF-a production was reduced by 77% and 95%, respectively. mRNA levels were measured by Northern blot analysis, performed 4 hours after LPS-stimulation using digoxigenin-labeled probes. There was no difference in IL-1B or TNF-a mRNA levels between cells stimulated with LPS with or without lung surfactant. Nitric oxide, IL-1B and TNF-a are known to be regulated at both the transcriptional and post-transcriptional levels. It is interesting that lung surfactant inhibits the production of all three pro-inflammatory molecules at the post-transcriptional level. This suggests that the post-transcriptional regulation of these three molecules may share a similar mechanism. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Lung Occupational Health Respiratory System Safety
• Keywords:
  Alveolar-cells Animal-studies Laboratory-animals Lung-function Pulmonary-function
• ISSN:
  1082-5589
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  48
• Issue:
  2
• NIOSHTIC Number:
  nn:20032619
• Citation:
  J Investig Med 2000 Mar; 48(2):200A
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  Journal of Investigative Medicine
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:c71ad64df9b416a97d62e8686cffe3cb5d9afe6009f6a7a38e9c17d356325777e3b604370cd68dd64ffb7ff5a51ed0e0473149c20b8cbcbca13b537ffd0b96ab
• Download URL:
  https://stacks.cdc.gov/view/cdc/186528/cdc_186528_DS1.pdf
• File Type:
  [PDF - 165.40 KB ]