Lung Surfactant Decreases IL-1 and TNF-A Production at the Post-Transcriptional Level in LPS-Stimulated Alveolar Macrophages

Details

• Personal Author:
  Bowman L ; Meighan T ; Rao KMK
• Description:
  We have shown previously that lung surfactant inhibits nitric oxide production at the post-transcriptional level in lipopolysaccharide(LPS)-stimulated rat alveolar macrophages (Miles et al., Am.J.Physiol. 276:Ll86, 1999). In this study, we examined the effect of lung surfactant (200 microg phospholipid/ml), isolated from rat lung lavage, on the production of two pro-inflammatory cytokines, IL-1B and TNF-a, by LPS-stimulated rat alveolar macrophages. After 22 h incubation with LPS plus lung surfactant, IL-1B and TNF-a production was reduced by 77% and 95%, respectively. mRNA levels were measured by Northern blot analysis, performed 4 hours after LPS-stimulation using digoxigenin-labeled probes. There was no difference in IL-1B or TNF-a mRNA levels between cells stimulated with LPS with or without lung surfactant. Nitric oxide, IL-1B and TNF-a are known to be regulated at both the transcriptional and post-transcriptional levels. It is interesting that lung surfactant inhibits the production of all three pro-inflammatory molecules at the post-transcriptional level. This suggests that the post-transcriptional regulation of these three molecules may share a similar mechanism. [Description provided by NIOSH]
• Subjects:
  Animals Laboratories Lung Occupational Health Respiratory System Safety
• Keywords:
  Alveolar Cells Animal Studies Laboratory Animals Lung Function Pulmonary Function
• ISSN:
  0892-6638
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  14
• Issue:
  4
• NIOSHTIC Number:
  nn:20021048
• Citation:
  FASEB J 2000 Mar; 14(4):A601
• CAS Registry Number:
  Nitric oxide (CAS RN 10102-43-9)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  The FASEB Journal
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:63ba7fe3b1e3385e16030a42ec77eceb56b6963cecc2f359de80a776a7aec467673c90290b71b75e431b0eb9ff67ddf06df08a63acb16d808527b56de885770b
• Download URL:
  https://stacks.cdc.gov/view/cdc/198622/cdc_198622_DS1.pdf
• File Type:
  [PDF - 335.91 KB ]