Antioxidant Balance and Free Radical Generation in Vitamin E Deficient Mice After Dermal Exposure to Cumene Hydroperoxide

Details

• Personal Author:
  Kadiiska MB ; Kisin ER ; Kommineni C ; Mason RP ; Shvedova AA
• Description:
  Organic peroxides are widely used in the chemical industry as initiators of oxidation for the production of polymers and fiber-reinforced plastics, in the manufacture of polyester resin coatings, and as antimicrobial agents in pharmaceuticals. Free radical production is considered to be one of the key factors contributing to skin tumor promotion by organic peroxides. In vitro experiments have demonstrated metal-catalyzed formation of alkoxyl, alkyl and aryl radicals in keratinocytes incubated with cumene hydroperoxide. The present study investigated in vivo tree radical generation in lipid extracts of mouse skin exposed to cumene hydroperoxicle. The electron spin resonance (ESR) spin-trapping technique was used to detect the formation of alfa-phenyl-N-tert-butylnitrone (PBN) radical adducts, following intradermal injection of 180 mg/kg PBN. It was found that 30 min after topical exposure, cumene hydroperoxide (12mmole/kg) induced free radical generation in the skin of female Balb/C mice (13-14 weeks old) kept for 10 weeks on vitamin E deficient diets. In contrast, no radical adducts were detected when cumene hydroperoxide was applied to the skin of mice fed a vitamin E sufficient diet. Important1y, levels of GSH and vitamin E in the skin of vitamin E deficient mice decreased 30% and 80%, respectively, compared to vitamin E sufficient controls. PBN adducts detected by ESR in vitamin E deficient mice provide direct evidence for in vivo tree radical generation in the skin after exposure to cumene hydroperoxide. [Description provided by NIOSH]
• Subjects:
  Animals Antioxidants Biopharmaceutics Environmental Exposure Free Radicals Laboratories Occupational Health Polymers Safety
• Keywords:
  Animal Studies Antioxidation Aryls Exposure Assessment Exposure Levels Free Radical Generation Free Radicals In Vitro Studies In Vivo Studies Laboratory Animals Organic Peroxides Oxidation Pharmaceuticals Skin Tumors

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• ISSN:
  1096-6080
• Document Type:
  Text
• Genre:
  Abstract or Summary
• Place as Subject:
  North Carolina ; OSHA Region 3 ; OSHA Region 4 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  49
• Volume:
  54
• Issue:
  1
• NIOSHTIC Number:
  nn:20021065
• Citation:
  Toxicologist 2000 Mar; 54(1):49
• CAS Registry Number:
  Cumene hydroperoxide (CAS RN 80-15-9)
• Federal Fiscal Year:
  2000
• Peer Reviewed:
  False
• Source Full Name:
  The Toxicologist. Society of Toxicology 39th Annual Meeting, March 19-23, 2000, Philadelphia, Pennsylvania
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:720313ab408b3f1d2ce4bcc66ef9c90f91796b17d8a94e66f6160990aa180fdf0658e9ba633ba47553476e38ddaee814600a76e13c61831a7714d47230286695
• Download URL:
  https://stacks.cdc.gov/view/cdc/198631/cdc_198631_DS1.pdf
• File Type:
  [PDF - 338.72 KB ]