Suppression of Phagocytic and Bactericidal Functions of Rat Alveolar Macrophages by the Organic Component of Diesel Exhaust Particles

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• Personal Author:
  Antonini JM ; Dong CC ; Ma JKH ; Ma JYC ; Roberts, Jennifer R. ; Yin XJ
• Description:
  Exposure to diesel exhaust particles (DEP) was shown to increase the susceptibility of the lung to bacterial infection in rats. In this study, the effects of DEP on alveolar macrophage (AM) phagocytic and bactericidal functions and cytokine secretion by AM and lymphocytes in response to Listeria monocytogenes infection were investigated in vitro and the roles of different DEP components in these processes were compared. Exposure to DEP or the organic extracts of DEP (eDEP) significantly decreased the phagocytosis and killing of L. monocytogenes by AM obtained from normal rats. Washed DEP (wDEP) also decreased AM phagocytosis and bacterial killing to a lesser extent, whereas carbon black (CB) reduced AM phagocytosis but had no significant effect on AM bactericidal activity. DEP or eDEP concentration-dependently suppressed L. monocytogenes-induced secretion of tumor necrosis factor-, interleukin (IL)-1, and IL-12 by AM and of IL-2 and interferon- by lymphocytes obtained from L. monocytogenes-infected rats, but augmented the AM secretion of IL-10. wDEP or CB, however, exerted little or no effect on these L. monocytogenes-induced cytokines. These results provide direct evidence that DEP, through the actions of organic components, suppresses AM phagocytic and bactericidal functions in vitro. Inhibition of AM phagocytic function and alterations of AM and lymphocyte cytokine secretion by DEP and DEP organic compounds may be implicated in the diminished AM bactericidal activity and the lymphatic arm of the host immune system, thus resulting in an suppressed pulmonary clearance of L. monocytogenes and an increased susceptibility of the lung to bacterial infection. [Description provided by NIOSH]
• Subjects:
  Animals Antigens Bacteria Blood Dust Hematologic Diseases Lung Lung Diseases Occupational Health Particulate Matter Respiratory System Respiratory Tract Diseases Safety Vehicle Emissions

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• Keywords:
  Animal-studies Bacterial-dusts Blood-cells Blood-disorders Diesel-emissions Immune-system-disorders Lung-disease Lung-disorders Particulate-dust Pulmonary-disorders Pulmonary-function Pulmonary-system-disorders

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• ISSN:
  1528-7394
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  70
• Issue:
  10
• NIOSHTIC Number:
  nn:20031865
• Citation:
  J Toxicol Environ Health A 2007 May; 70(10):820-828
• Contact Point Address:
  Xuejun J. Yin, MD, PhD, School of Medicine, West Virginia University, 1 Medical Center Drive, Morgantown, WV 26506-9203
• Email:
  xyin@hsc.wvu.edu
• Federal Fiscal Year:
  2007
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Toxicology and Environmental Health, Part A: Current Issues
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:1bf5bd266b2877f2d8a97bda2ca6a17b5e84e50ed3cc8a12d988647386fbf457c94ded69a27fa977b45b9c558851b914c1427a72a1eeda7853016777493315ca
• Download URL:
  https://stacks.cdc.gov/view/cdc/185989/cdc_185989_DS1.pdf
• File Type:
  [PDF - 350.63 KB ]