Fluticasone Propionate and Pentamidine Isethionate Reduce Airway Hyperractivity, Pulmonary Eosinophilia and Pulmonary Dendritic Cell Response in a Guinea Pig Model of Asthma

Details

• Personal Author:
  Fedan, Jeffery S. ; Lawrence TE ; Millecchia LL
• Description:
  In this study, we examined the effects of fluticasone propionate (FP) and pentamidine isethionate (PI) on antigen-induced lung inflammation and airway hyperreactivity in guinea pigs. Male guinea pigs were sensitized on days 0 and 14 with 10 micrograms of ovalbumin (OVA) plus 1 mg of Al(OH)3. On day 21, animals were challenged with a 2% OVA aerosol inhalation until they developed pulmonary obstruction. Animals were treated with aerosol inhalation of FP (2 ml of 0.5 mg/ml, five consecutive doses at 12-hr intervals with the last dose given 6 hr before OVA challenge) or PI (30 mg/ml for 30 min 1 hr before OVA challenge), and control animals received no drug before OVA challenge. Airway reactivity to methacholine (MCh) was assessed before sensitization and 18 hr after OVA challenge. At 18 hr after challenge, histological sections of trachea and lung were examined for eosinophil, dendritic cell (DC) and macrophage cell densities in the airways. In control animals, OVA evoked airway hyperreactivity to MCh in conjunction with pulmonary eosinophilia and increases in DC prevalence in the trachea and bronchi. Treatment with FP or PI abolished the OVA-induced hyperresponsiveness and significantly reduced the OVA-induced increases in eosinophils and DCs in the airways. FP and PI had no effect on saline-treated animals. Our study indicates that both inhaled FP and inhaled PI reduce antigen-induced airway hyperreactivity and pulmonary inflammation in guinea pigs. The results also suggest that the DC is a target of the anti-inflammatory effects of these drugs in the airways. [Description provided by NIOSH]
• Subjects:
  Animals Antigens Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Animal-studies Laboratory-animals Lung-burden Lung-disease Lung-irritants Pulmonary-congestion Pulmonary-disorders Pulmonary-function
• ISSN:
  0022-3565
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Pages in Document:
  222-227
• Volume:
  284
• Issue:
  1
• NIOSHTIC Number:
  nn:20025210
• Citation:
  J Pharmacol Exp Ther 1998 Jan; 284(1):222-227
• Federal Fiscal Year:
  1998
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Pharmacology and Experimental Therapeutics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:750afe2b71f025676ed65882be34f046674ccfd53e46d67f23cf52337bac24e0d8ffaae2336b0bd66d068eadd97dc3f1ed481a4a64150acda5ae9de4b77e2371
• Download URL:
  https://stacks.cdc.gov/view/cdc/196681/cdc_196681_DS1.pdf
• File Type:
  [PDF - 1.57 MB ]