Pulmonary Dendritic Cell Distribution and Prevalence in Guinea Pig Airways: Effect of Ovalbumin Sensitization and Challenge

Details

• Personal Author:
  Fedan, Jeffery S. ; Frazer DG ; Lawrence TE ; Millecchia LL
• Description:
  We characterized the localization and prevalence of dendritic cells (DC) in guinea pig airways before and after s.c. sensitization and aerosol challenge with ovalbumin (OVA). DC, eosinophils, macrophages, T cells and B cells in lung and trachea were identified and quantified in frozen sections using monoclonal antibodies and computer-assisted image analysis. Airway reactivity of conscious animals to inhaled methacholine was examined. In unsensitized animals, DC were localized primarily within the lamina propria of the trachea and bronchi, in the submucosa of the trachea and in the adventitia of the bronchi. In contrast to reported studies on rats, few DC were noted in the epithelium. After OVA challenge, sensitized animals demonstrated an early obstructive response and a late-phase response that was well developed by 18 hr. Challenge with OVA increased DC prevalence in the lamina propria and submucosa of the trachea and in the lamina propria and adventitia of the bronchi. There was widespread eosinophilia throughout the airways, but no changes in B cells or T cells were evident. Macrophages were increased in the epithelium of both OVA-treated and saline-treated animals. At 18 hr after challenge, sensitized guinea pigs but not saline-treated controls were hyperreactive to inhaled methacholine. Except for macrophages, none of these effects were observed after saline treatment. Our findings indicate that inflammation in the airways of OVA-sensitized guinea pigs involves infiltration of DC, which is seen at the time animals are hyperreactive to inhaled methacholine. [Description provided by NIOSH]
• Subjects:
  Animals Antigens Laboratories Lung Lung Diseases Occupational Health Respiratory System Respiratory Tract Diseases Safety
• Keywords:
  Animal-studies Laboratory-animals Lung-burden Lung-disease Lung-irritants Pulmonary-congestion Pulmonary-disorders Pulmonary-function Respiratory-system-disorders
• ISSN:
  0022-3565
• Document Type:
  Text
• Genre:
  Journal Article
• Place as Subject:
  OSHA Region 3 ; West Virginia
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  HELD - Health Effects Laboratory Division
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  282
• Issue:
  2
• NIOSHTIC Number:
  nn:20033928
• Citation:
  J Pharmacol Exp Ther 1997 Aug; 282(2):995-1004
• Contact Point Address:
  Jeffrey S. Fedan, NIOSH, 1095 Willowdale Road, Morgantown, WV 26505
• Federal Fiscal Year:
  1997
• Peer Reviewed:
  True
• Source Full Name:
  Journal of Pharmacology and Experimental Therapeutics
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:b483262b47d7a4a9e34289c1d2e2e2bf11ee445103a7656d373ca741a91f48d569f54903a651bfab1baf73dbe9988551924b4bf8eccab0844514a52699532dad
• Download URL:
  https://stacks.cdc.gov/view/cdc/189327/cdc_189327_DS1.pdf
• File Type:
  [PDF - 360.84 KB ]