Development, Validation and Use of a Fluorescent Covalent Microsphere Immunoassay (FCMIA) for the Measurement of IgG Antibodies to Bacillus anthracis Protective Antigen in Human Sera

Details

• Personal Author:
  Biagini, Raymond E. ; MacKenzie, Barbara A. ; Quinn CP ; Sammons DL ; Smith JP ; Snawder, John E. ; Striley CAF
• Description:
  At the request of the Environmental Protection Agency, we performed immuno­logical biological monitoring for exposure to Bacillus anthracis (Ba) on workers conducting environmental sampling in response to the October, 2001 terror attack at the American Media, Inc., building In Boca Raton, FL. We developed, validated and used a fluorescent covalent microsphere immunoassay (FCMIA) to measure anti-Ba-protective antigen (PA) IgG in workers' sera. The FCMIA gave 4 positive anti-PA IgG results. The positive sera were the pre-entry and follow-up samples of two workers who had received their last dose of anthrax vaccine In 2000. We also compared the FCMIA to a Centers for Disease Control standardized enzyme-linked immunosorbent assay (ELISA) for anti-PA IgG (Emerg Infect Dis 8:1103-10, 2002). A high positive correlation (R=0.923, P <0.001) was observed when 20 other samples were co-analyzed for anti-PA IgG in a double-masked analysis using FCMIA and ELISA. [Description provided by NIOSH]
• Subjects:
  Antibodies Environmental Exposure Environmental Monitoring Environmental Pollution Hematologic Tests Occupational Health Safety
• Keywords:
  Antibody-response Bacteria Bacterial-disease Blood-analysis Environmental-contamination Environmental-exposure Environmental-hazards Exposure-assessment Exposure-levels Serological-techniques
• ISSN:
  0065-7727
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Georgia ; Ohio ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  226
• NIOSHTIC Number:
  nn:20031844
• Citation:
  Abstr Pap - Am Chem Soc 2003 Sep; 226(Pt 1):U93 066-AGRO
• Contact Point Address:
  Biomonitoring and Health Assessment Branch, CDC/NIOSH, 4676 Columbia Parkway, Mailstop C-26, Cincinnati, OH 45226
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  False
• Part Number:
  1
• Source Full Name:
  Abstracts of Papers - American Chemical Society
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:5a6910bbe36e19e2522dbda28e2107cb3526b2a2d3e68770aa3c3010a40f0194a969a6e09af264569351d6676d6f521b5f624a46637781c16a0b641fad318a5e
• Download URL:
  https://stacks.cdc.gov/view/cdc/185981/cdc_185981_DS1.pdf
• File Type:
  [PDF - 82.60 KB ]