Multiplexed Analyses of Serum Antibodies to Bacillus anthracis Toxins from Cases with Cutaneous Anthrax in the Former Soviet Republic of Kazakhstan

Details

• Personal Author:
  Biagini, Raymond E. ; MacKenzie, Barbara A. ; Quinn CP ; Robertson SK ; Sammons DL ; Smith JP ; Snawder, John E. ; Striley CAF ; Wilkins PP
• Description:
  Bacillus anthracis (Ba) exotoxins, lethal factor (IF) and protective antigen (PA) are immunogenic, and exposure may be evaluated by measurement of specific antibodies by multiplexed fluorescence covalent microbead Immunoassay (FCMIA). Using sera from individuals with "confirmed" cutaneous cases of (N=7) of anthrax from the former Soviet Republic of Kazakhstan (Kaz) and 8 donor controls we found 47.9 +/- 37.4 and 19.0 +/- 16.3, 1.00 +/- 0.98 and 0.14 +/- 0.12 microg/ml anti-LF lgG and anli-PA IgG, respectively (Kaz and control; mean +/- Standard Deviation (SD]; both P<0.001). For anti-LF 19M and anti-PA 19M, we found 1107 +/- 999 and 265 +/- 174, 33.5 +/- 29.7 and 9.6 +/- 6.0 (Kaz and control; standard sera equivalent dilution +/- SD; P<0.001), respectively, These data Indicate that cutaneous anthrax Infection can induce significant levels of anti-LF and anti-PA IgG and IgM which can be measured by multiplexed FCMIA. [Description provided by NIOSH]
• Subjects:
  Antibodies Dermatitis Environmental Monitoring Hematologic Tests Occupational Health Safety Skin Skin Absorption
• Keywords:
  Antibody-response Bacteria Bacterial-disease Blood-analysis Exposure-assessment Exposure-levels Serological-techniques Skin-absorption Skin-exposure
• ISSN:
  0065-7727
• Document Type:
  Text
• Genre:
  Abstract or Summary ; Proceedings
• Place as Subject:
  Georgia ; Ohio ; OSHA Region 4 ; OSHA Region 5
• CIO:
  National Institute for Occupational Safety and Health (NIOSH)
• Division:
  DART - Division of Applied Research and Technology
• Topic:
  Workplace Safety & Health
• Location:
  North America
• Volume:
  226
• NIOSHTIC Number:
  nn:20031843
• Citation:
  Abstr Pap - Am Chem Soc 2003 Sep; 226(Pt 1):U90 044-AGRO
• Contact Point Address:
  Deborah L. Sammons, Biomonitoring and Health Assessment Branch, CDC/NIOSH, 4676 Columbia Parkway, Mailstop C-26, Cincinnati, OH 45226
• Email:
  dls7@cdc.gov
• Federal Fiscal Year:
  2003
• Peer Reviewed:
  False
• Part Number:
  1
• Source Full Name:
  Abstracts of Papers - American Chemical Society
• Collection(s):
  National Institute for Occupational Safety and Health
• Main Document Checksum:
  urn:sha-512:ac2a0c1b3505bfe70007b5893503048af1511bd8734a96663c19fa65551ccdfb7123203c9921564d3633a5b6cb8bed14f65d50769448974828daa984c0167437
• Download URL:
  https://stacks.cdc.gov/view/cdc/185980/cdc_185980_DS1.pdf
• File Type:
  [PDF - 84.55 KB ]