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i

Methodology to measure the transient effect of occlusion on skin penetration and stratum corneum hydration in vivo.



Details

  • Personal Author:
  • Description:
    The absorption of hexyl-nicotine (HN) to the ventral forearm surface of the skin of human volunteers was examined following random assignment to one of three pretreatments: protocol 1, no pretreatment; protocol 2, occlusion for 30 minutes using a small polypropylene chamber; and protocol 3, 30 minutes occlusion as before plus subsequent exposure to ambient conditions for 1 hour. The effect of these treatments on percutaneous absorption and skin hydration were made using laser Doppler velocimetry (LDV) and the dielectric probe technique. The 30 minute period of occlusion shortened significantly both the time of onset of LDV detected response to HN and the time to peak response when compared to the no treatment control. The magnitude of the LDV response to HN and the area under the response versus time curve were significantly increased. Under treatment where the occluded site was exposed to ambient conditions for 1 hour post occlusion before NH dosing, blood flow responses were not significantly different from controls. The stratum corneum water content values showed the same pattern of behavior. Protocol 3 was indistinguishable from control levels whereas protocol 2 caused the horny layer water content to be significantly elevated. These findings probably reflect different intersubject skin dehydration rates following a period of occlusion. The authors suggest that extending the period of ambient exposure would reduce to some extent the variability in the results. [Description provided by NIOSH]
  • Content Notes:
    in NTRL, no pdf
  • Subjects:
  • Keywords:
  • Series:
    Grant Final Reports
  • Document Type:
    Text
  • Funding:
    Grant
  • Genre:
    Final Grant Report
  • Place as Subject:
  • CIO:
    National Institute for Occupational Safety and Health (NIOSH)
  • Division:
    OEP - Office of Extramural Programs
  • Topic:
    Workplace Safety & Health
  • Location:
    North America
  • Pages in Document:
    1-17
  • NIOSHTIC Number:
    nn:00182771
  • NTIS Accession Number:
    PB89129597
  • Citation:
    Departments of Pharmacy, Pharmaceutical Chemistry and Dermatology, Schools of Pharmacy and Medicine, University of California, San Francisco, California, 1987 Dec; :1-17
  • Contact Point Address:
    Pharmacy University of California 926 Medical Sciences Building San Francisco, Calif 94143
  • Federal Fiscal Year:
    1988
  • NORA Priority Area:
    Disease and Injury: Allergic and Irritant Dermatitis
  • Performing Organization:
    University of California San Francisco, San Francisco, California
  • Peer Reviewed:
    False
  • Start Date:
    19840928
  • Source Full Name:
    Departments of Pharmacy, Pharmaceutical Chemistry and Dermatology, Schools of Pharmacy and Medicine, University of California, San Francisco, California
  • End Date:
    19871231
  • Collection(s):
  • Main Document Checksum:
    urn:sha-512:7dc847c09aedf140bc731323e8446b4703641ae07d6ad9e7b7238120dab30b8f7dcaa9b938d34d035f87873a0fe7fec262d5d13dcaf290925445e82b6de3b57d
  • Download URL:
  • File Type:
    Filetype[PDF - 168.02 KB ]
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